Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1988 Nov;26(11):2357–2360. doi: 10.1128/jcm.26.11.2357-2360.1988

Improved selective medium for the isolation of Treponema hyodysenteriae.

R A Kunkle 1, J M Kinyon 1
PMCID: PMC266892  PMID: 3235663

Abstract

An agar medium with improved selection for Treponema hyodysenteriae was developed. Cultures of T. hyodysenteriae and T. innocens, feces from 11 clinically normal pigs, and colonic contents from 6 pigs with gross lesions consistent with swine dysentery were diluted in phosphate-buffered saline and plated on Trypticase soy agar (BBL Microbiology Systems, Cockeysville, Md.) with 5% citrated bovine blood (TSA), TSA with 400 micrograms of spectinomycin per ml (TSA-S400), TSA-S400 with 25 micrograms each of colistin and vancomycin per ml, and TSA with 5% pig feces extract and five antimicrobial agents (spiramycin, rifampin, vancomycin, colistin, and spectinomycin) (BJ). Viable numbers of T. hydodysenteriae grown on BJ were virtually identical to those for TSA, TSA-S400, and TSA-S400 with colistin and vancomycin. Pure cultures of four isolates of T. hyodysenteriae and three isolates of T. innocens were sustained through six subcultures on BJ. Fecal floras were completely inhibited on BJ for 14 of 17 fecal samples from both groups of pigs. A total of 461 colonic specimens from naturally occurring cases of porcine intestinal disease were plated on TSA-S400 and BJ. T. hyodysenteriae was isolated on both TSA-S400 and BJ for 69 specimens and on BJ alone for an additional 19 specimens.

Full text

PDF
2357

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adachi Y., Kashiwazaki M., Kume T. Comparison of antigenic properties among various strains of Treponema hyodysenteriae. Zentralbl Bakteriol Orig A. 1979 Dec;245(4):527–533. [PubMed] [Google Scholar]
  2. Burrows M. R., Lemcke R. M. Identification of Treponema hyodysenteriae by a rapid slide agglutination test. Vet Rec. 1981 Feb 28;108(9):187–189. doi: 10.1136/vr.108.9.187. [DOI] [PubMed] [Google Scholar]
  3. Griffin R. M. Sensitivity in vitro to dimetridazole of treponemes associated with swine dysentery. Vet Rec. 1979 Jan 27;104(4):73–74. doi: 10.1136/vr.104.4.73. [DOI] [PubMed] [Google Scholar]
  4. Harris D. L., Kinyon J. M., Mullin M. T., Glock R. D. Isolation and propagation of spirochetes from the colon of swine dysentery affected pigs. Can J Comp Med. 1972 Jan;36(1):74–76. [PMC free article] [PubMed] [Google Scholar]
  5. Hunter D., Wood T. An evaluation of the API ZYM system as a means of classifying spirochaetes associated with swine dysentery. Vet Rec. 1979 Apr 28;104(17):383–384. doi: 10.1136/vr.104.17.383. [DOI] [PubMed] [Google Scholar]
  6. Jenkins E. M., Adderley J., Rayner J., Hurdle C. Use of oral vaccines in attempts to prevent swine dysentery. Vet Med Small Anim Clin. 1979 Dec;74(12):1785–1790. [PubMed] [Google Scholar]
  7. Jenkinson S. R., Wingar C. R. Selective medium for the isolation of Treponema hyodysenteriae. Vet Rec. 1981 Oct 24;109(17):384–385. doi: 10.1136/vr.109.17.384. [DOI] [PubMed] [Google Scholar]
  8. Joens L. A., Glock R. D. Experimental infection in mice with Treponema hyodysenteriae. Infect Immun. 1979 Aug;25(2):757–760. doi: 10.1128/iai.25.2.757-760.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Joens L. A., Kinyon J. M. Isolation of Treponema hyodysenteriae from wild rodents. J Clin Microbiol. 1982 Jun;15(6):994–997. doi: 10.1128/jcm.15.6.994-997.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kinyon J. M., Harris D. L., Glock R. D. Enteropathogenicity of various isolates of Treponema hyodysenteriae. Infect Immun. 1977 Feb;15(2):638–646. doi: 10.1128/iai.15.2.638-646.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kitai K., Kashiwazaki M., Adachi Y., Kume T., Arakawa A. In vitro activity of 39 antimicrobial agents against Treponema hyodysenteriae. Antimicrob Agents Chemother. 1979 Mar;15(3):392–395. doi: 10.1128/aac.15.3.392. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kunkle R. A., Harris D. L., Kinyon J. M. Autoclaved liquid medium for propagation of Treponema hyodysenteriae. J Clin Microbiol. 1986 Oct;24(4):669–671. doi: 10.1128/jcm.24.4.669-671.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lemcke R. M., Burrows M. R. A disc growth-inhibition test for differentiating Treponema hyodysenteriae from other intestinal spirochaetes. Vet Rec. 1979 Jun 16;104(24):548–551. doi: 10.1136/vr.104.24.548. [DOI] [PubMed] [Google Scholar]
  14. Liven E. Prevalence of Treponema hyodysenteriae in healthy pigs. Acta Vet Scand. 1979;20(3):387–395. doi: 10.1186/BF03546600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Olson L. D., Fales W. H. Comparison of stained smears and culturing for identification of Treponema hyodysenteriae. J Clin Microbiol. 1983 Oct;18(4):950–955. doi: 10.1128/jcm.18.4.950-955.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Picard B., Lariviere S., Saheb S. A. Etude comparative des caractères biochimiques de tréponèmes hémolytiques isolés du porc. Can J Microbiol. 1980 Aug;26(8):985–991. [PubMed] [Google Scholar]
  17. Raynaud J. P., Brunault G., Philippe J. Swine dysentery. Comparison of experimental diseases produced by infection with colonic mucosa or with Treponema hyodysenteriae, French strains, and of "natural" disease. Ann Rech Vet. 1980;11(1):68–87. [PubMed] [Google Scholar]
  18. Smibert R. M., Claterbaugh R. L., Jr A chemically defined medium for Treponema strain PR-7 isolated from the intestine of a pig with swine dysentery. Can J Microbiol. 1972 Jul;18(7):1073–1078. doi: 10.1139/m72-166. [DOI] [PubMed] [Google Scholar]
  19. Smit H. F., Jongerius J. M. Swine dysentery diagnosis: to isolate or to fluoresce? Vet Rec. 1982 Oct 9;111(15):343–343. doi: 10.1136/vr.111.15.343. [DOI] [PubMed] [Google Scholar]
  20. Songer J. G., Kinyon J. M., Harris D. L. Selective medium for isolation of Treponema hyodysenteriae. J Clin Microbiol. 1976 Jul;4(1):57–60. doi: 10.1128/jcm.4.1.57-60.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sutter V. L., Sugihara P. T., Finegold S. M. Rifampin-blood-agar as a selective medium for the isolation of certain anaerobic bacteria. Appl Microbiol. 1971 Nov;22(5):777–780. doi: 10.1128/am.22.5.777-780.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Szynkiewicz Z. M., Binek M. Evaluation of selective media for primary isolation of Treponema hyodysenteriae and Treponema innocens. Comp Immunol Microbiol Infect Dis. 1986;9(1):71–77. doi: 10.1016/0147-9571(86)90077-9. [DOI] [PubMed] [Google Scholar]
  23. Taylor D. J., Alexander T. J. The production of dysentery in swine by feeding cultures containing a spirochaete. Br Vet J. 1971 Nov;127(11):58–61. doi: 10.1016/s0007-1935(17)37282-2. [DOI] [PubMed] [Google Scholar]
  24. Wilcock B. P., Olander H. J. Studies on the pathogenesis of swine dysentery. I. Characterization of the lesions in colons and colonic segments inoculated with pure cultures or colonic content containing Treponema hyodysenteriae. Vet Pathol. 1979 Jul;16(4):450–465. doi: 10.1177/030098587901600409. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES