Abstract
The clinical need to rapidly and correctly differentiate beta-hemolytic streptococci into Lancefield groups has prompted the development of commercially available rapid agglutination techniques. A modified Streptex (Wellcome Diagnostics, Research Triangle Park, N.C.) technique and the PathoDx latex Strep Grouping Kit (Diagnostic Products Corporation, Los Angeles, Calif.) technique were applied to 220 strains of beta-hemolytic streptococci that were serologically grouped by standard techniques. Agreements between standard and modified Streptex and PathoDx techniques were 99.1 and 100%, respectively. Modified Streptex produced a false-negative for one group G isolate and a weak-positive reaction for a group G reagent with a nongroupable isolate. Sixty-five strains representative of bacteria that may be found in the posterior pharynx in concentrations high enough to cause potential reactions with antigen detection reagents were tested with Streptex and PathoDx reagents. No cross-reactions were observed with any reagent tested when challenged with these 65 strains. When combined with colonial morphology and hemolytic reaction, both modified Streptex and PathoDx were rapid, specific tests for identifying streptococci, with PathoDx being slightly faster.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bixler-Forell E., Martin W. J., Moody M. D. Clinical evaluation of the improved streptex method for grouping streptococci. Diagn Microbiol Infect Dis. 1984 Apr;2(2):113–118. doi: 10.1016/0732-8893(84)90006-3. [DOI] [PubMed] [Google Scholar]
- Burdash N. M., West M. E., Newell R. T., Teti G. Group identification of streptococci. Evaluation of three rapid agglutination methods. Am J Clin Pathol. 1981 Dec;76(6):819–822. doi: 10.1093/ajcp/76.6.819. [DOI] [PubMed] [Google Scholar]
- Castle D., Kessock-Philip S., Easmon C. S. Evaluation of an improved Streptex kit for the grouping of beta-haemolytic streptococci by agglutination. J Clin Pathol. 1982 Jul;35(7):719–722. doi: 10.1136/jcp.35.7.719. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chang G. T., Ellner P. D. Evaluation of slide agglutination methods for identifying group D streptococci. J Clin Microbiol. 1983 May;17(5):804–806. doi: 10.1128/jcm.17.5.804-806.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Engel H. W., van Silfhout A. Simplified coagglutination test for serological grouping of beta-hemolytic streptococci. J Clin Microbiol. 1981 Sep;14(3):252–255. doi: 10.1128/jcm.14.3.252-255.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Facklam R. R., Cooksey R. C., Wortham E. C. Evaluation of commercial latex agglutination reagents for grouping streptococci. J Clin Microbiol. 1979 Nov;10(5):641–646. doi: 10.1128/jcm.10.5.641-646.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hamilton J. R. Comparison of Meritec-Strep with Streptex for direct colony grouping of beta-hemolytic streptococci from primary isolation and subculture plates. J Clin Microbiol. 1988 Apr;26(4):692–695. doi: 10.1128/jcm.26.4.692-695.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Slifkin M., Interval G. Serogrouping single colonies of beta-hemolytic streptococci from primary throat culture plates with nitrous acid extraction and Phadebact streptococcal reagents. J Clin Microbiol. 1980 Oct;12(4):541–545. doi: 10.1128/jcm.12.4.541-545.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Slifkin M., Pouchet-Melvin G. R. Evaluation of three commercially available test products for serogrouping beta-hemolytic streptococci. J Clin Microbiol. 1980 Mar;11(3):249–255. doi: 10.1128/jcm.11.3.249-255.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wellstood S. Evaluation of Phadebact and Streptex Kits for rapid grouping of streptococci directly from blood cultures. J Clin Microbiol. 1982 Feb;15(2):226–230. doi: 10.1128/jcm.15.2.226-230.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]