Abstract
Culture and fluorescent-antibody methods for detection of Bordetella species were evaluated by two state public health laboratories. Field-inoculated plates of Regan-Lowe agar medium were most useful if incubation was initiated on the day of collection. Regan-Lowe and Bordet-Gengou media were comparable for subculturing nasopharyngeal specimens that were transported and enriched in half-strength Regan-Lowe agar. Maximum sensitivity was achieved when the media were used in parallel. Fluorescent-antibody-stained smears of nasopharyngeal specimens were more sensitive for detection of Bordetella pertussis than for detection of Bordetella parapertussis. The fluorescent-antibody method, however, was too insensitive for use without culture.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ahmad F., Calder M. A. Isolation of Bordetella pertussis: benefits of using both Bordet-Gengou and charcoal agar media. J Clin Pathol. 1984 Sep;37(9):1071–1072. doi: 10.1136/jcp.37.9.1071. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hoppe J. E., Vogl R. Comparison of three media for culture of Bordetella pertussis. Eur J Clin Microbiol. 1986 Jun;5(3):361–363. doi: 10.1007/BF02017801. [DOI] [PubMed] [Google Scholar]
- Hoppe J. E., Weiss A. Recovery of Bordetella pertussis from four kinds of swabs. Eur J Clin Microbiol. 1987 Apr;6(2):203–205. doi: 10.1007/BF02018215. [DOI] [PubMed] [Google Scholar]
- Hoppe J. E., Wörz S., Botzenhart K. Comparison of specimen transport systems for Bordetella pertussis. Eur J Clin Microbiol. 1986 Dec;5(6):671–673. doi: 10.1007/BF02013299. [DOI] [PubMed] [Google Scholar]
- Kurzynski T. A., Boehm D. M., Rott-Petri J. A., Schell R. F., Allison P. E. Antimicrobial susceptibilities of Bordetella species isolated in a Multicenter Pertussis Surveillance Project. Antimicrob Agents Chemother. 1988 Jan;32(1):137–140. doi: 10.1128/aac.32.1.137. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Regan J., Lowe F. Enrichment medium for the isolation of Bordetella. J Clin Microbiol. 1977 Sep;6(3):303–309. doi: 10.1128/jcm.6.3.303-309.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]