Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1988 Dec;26(12):2667–2668. doi: 10.1128/jcm.26.12.2667-2668.1988

Microtiter method for MIC testing with spherule-endospore-phase Coccidioides immitis.

R F Hector 1, B L Zimmer 1, D Pappagianis 1
PMCID: PMC266970  PMID: 3230142

Abstract

A method was developed for susceptibility testing with spherule-endospore-phase Coccidioides immitis by using a microtiter format. Isolated endospores were used to inoculate wells containing modified Converse medium with various concentrations of azole or nikkomycin antifungal substances which then were sealed with an acetate film. The plate was incubated at 37 degrees C with shaking for 96 h, after which the control wells had visible turbidity and endpoints were discernible. Microscopic examination revealed that both control and treatment wells maintained cells predominantly in the spherule-endospore phase of growth.

Full text

PDF
2667

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Becker J. M., Covert N. L., Shenbagamurthi P., Steinfeld A. S., Naider F. Polyoxin D inhibits growth of zoopathogenic fungi. Antimicrob Agents Chemother. 1983 Jun;23(6):926–929. doi: 10.1128/aac.23.6.926. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CONVERSE J. L. Effect of physico-chemical environment of spherulation of Coccidioides immitis in a chemically defined medium. J Bacteriol. 1956 Dec;72(6):784–792. doi: 10.1128/jb.72.6.784-792.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CONVERSE J. L. Effect of surface active agents on endosporulation of Coccidioides immitis in a chemically defined medium. J Bacteriol. 1957 Jul;74(1):106–107. doi: 10.1128/jb.74.1.106-107.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CONVERSE J. L. Growth of spherules of Coccidioides immitis in a chemically defined liquid medium. Proc Soc Exp Biol Med. 1955 Dec;90(3):709–711. doi: 10.3181/00379727-90-22144. [DOI] [PubMed] [Google Scholar]
  5. Collins M. S., Pappagianis D. Uniform susceptibility of various strains of Coccidioides immitis to amphotericin B. Antimicrob Agents Chemother. 1977 Jun;11(6):1049–1055. doi: 10.1128/aac.11.6.1049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Converse J. L., Besemer A. R. NUTRITION OF THE PARASITIC PHASE OF COCCIDIOIDES IMMITIS IN A CHEMICALLY DEFINED LIQUID MEDIUM. J Bacteriol. 1959 Aug;78(2):231–239. doi: 10.1128/jb.78.2.231-239.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dixon D., Shadomy S., Shadomy H. J., Espinel-Ingroff A., Kerkering T. M. Comparison of the in vitro antifungal activities of miconazole and a new imidazole, R41,400. J Infect Dis. 1978 Aug;138(2):245–248. doi: 10.1093/infdis/138.2.245. [DOI] [PubMed] [Google Scholar]
  8. Graybill J. R., Sun S. H., Ahrens J. Treatment of murine coccidioidal meningitis with fluconazole (UK 49,858). J Med Vet Mycol. 1986 Apr;24(2):113–119. doi: 10.1080/02681218680000171. [DOI] [PubMed] [Google Scholar]
  9. Hector R. F., Pappagianis D. Inhibition of chitin synthesis in the cell wall of Coccidioides immitis by polyoxin D. J Bacteriol. 1983 Apr;154(1):488–498. doi: 10.1128/jb.154.1.488-498.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hoeprich P. D., Huston A. C. Susceptibility of coccidioides immitis, Candida albicans, and Cryptococcus neoformans to amphotericin B, flucytosine, and clotrimazole. J Infect Dis. 1975 Aug;132(2):133–141. doi: 10.1093/infdis/132.2.133. [DOI] [PubMed] [Google Scholar]
  11. Huppert M., Pappagianis D., Sun S. H., Gleason-Jordan I., Collins M. S., Vukovich K. R. Effect of amphotericin B and rifampin against Coccidioides immitis in vitro and in vivo. Antimicrob Agents Chemother. 1976 Mar;9(3):406–413. doi: 10.1128/aac.9.3.406. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LEVINE H. B., COBB J. M., SMITH C. E. Immunity to coccidioi-domycosis induced in mice by purified spherule, arthrospore, and mycelial vaccines. Trans N Y Acad Sci. 1960 Apr;22:436–449. doi: 10.1111/j.2164-0947.1960.tb00711.x. [DOI] [PubMed] [Google Scholar]
  13. Levine H. B., Cobb J. M. Oral therapy for experimental coccidioidomycosis with R41 400 (ketoconazole), a new imidazole. Am Rev Respir Dis. 1978 Oct;118(4):715–721. doi: 10.1164/arrd.1978.118.4.715. [DOI] [PubMed] [Google Scholar]
  14. Levine H. B., Stevens D. A., Cobb J. M., Gebhardt A. E. Miconazole in coccidioidomycosis. I. Assays of activity in mice and in vitro. J Infect Dis. 1975 Oct;132(4):407–414. doi: 10.1093/infdis/132.4.407. [DOI] [PubMed] [Google Scholar]
  15. Stevens D. A., Levine H. B., Deresinski S. C. Miconazole in coccidiodomycosis. II. Therapeutic and pharmacologic studies in man. Am J Med. 1976 Feb;60(2):191–202. doi: 10.1016/0002-9343(76)90428-9. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES