Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1989 Oct;27(10):2324–2327. doi: 10.1128/jcm.27.10.2324-2327.1989

Antibody response to recombinant lambda gt11 fusion proteins in cytomegalovirus infection.

M P Landini 1, T Lazzarotto 1, A Ripalti 1, M X Guan 1, M La Placa 1
PMCID: PMC267017  PMID: 2555392

Abstract

Significant epitopes of two of the major cytomegalovirus antigens, a nonstructural DNA-binding protein of 52 kilodaltons (kDa) and a structural phosphoprotein of 150 kDa, expressed as fusion proteins with the beta-galactosidase, were induced in Escherichia coli after infection with recombinant lambda gt11 clones. The epitopes were then used in immunoblotting to assay specific immunoglobulin G (IgG) and IgM in several groups of sera from long-term seropositive subjects and from patients undergoing primary or secondary virus infection. The data obtained showed that IgM reacting with the 52-kDa nonstructural antigen are linked to primary virus infection and can therefore be considered a serological marker of this infection.

Full text

PDF
2324

Images in this article

2326Image
on p.2326

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Betts R. F., Schmidt S. G. Cytolytic IgM antibody to cytomegalovirus in primary cytomegalovirus infection in humans. J Infect Dis. 1981 Jun;143(6):821–826. doi: 10.1093/infdis/143.6.821. [DOI] [PubMed] [Google Scholar]
  2. Chou S., Kim D. Y., Scott K. M., Sewell D. L. Immunoglobulin M to cytomegalovirus in primary and reactivation infections in renal transplant recipients. J Clin Microbiol. 1987 Jan;25(1):52–55. doi: 10.1128/jcm.25.1.52-55.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cremer N. E., Cossen C. K., Shell G. R., Pereira L. Antibody response to cytomegalovirus polypeptides captured by monoclonal antibodies on the solid phase in enzyme immunoassays. J Clin Microbiol. 1985 Apr;21(4):517–521. doi: 10.1128/jcm.21.4.517-521.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gibson W. Protein counterparts of human and simian cytomegaloviruses. Virology. 1983 Jul 30;128(2):391–406. doi: 10.1016/0042-6822(83)90265-9. [DOI] [PubMed] [Google Scholar]
  5. Griffiths P. D., Stagno S., Pass R. F., Smith R. J., Alford C. A., Jr Infection with cytomegalovirus during pregnancy: specific IgM antibodies as a marker of recent primary infection. J Infect Dis. 1982 May;145(5):647–653. doi: 10.1093/infdis/145.2.647. [DOI] [PubMed] [Google Scholar]
  6. Jahn G., Scholl B. C., Traupe B., Fleckenstein B. The two major structural phosphoproteins (pp65 and pp150) of human cytomegalovirus and their antigenic properties. J Gen Virol. 1987 May;68(Pt 5):1327–1337. doi: 10.1099/0022-1317-68-5-1327. [DOI] [PubMed] [Google Scholar]
  7. Kampa D., Schmitz H., Doerr H. W., Luthardt T., Hillemanns H. G., Wurtele A. IgM antibodies to cytomegalovirus during pregnancy. Arch Virol. 1977;53(3):177–184. doi: 10.1007/BF01314662. [DOI] [PubMed] [Google Scholar]
  8. Landini M. P., Michelson S. Human cytomegalovirus proteins. Prog Med Virol. 1988;35:152–185. [PubMed] [Google Scholar]
  9. Landini M. P., Mirolo G., Coppolecchia P., Re M. C., La Placa M. Serum antibodies to individual cytomegalovirus structural polypeptides in renal transplant recipients during viral infection. Microbiol Immunol. 1986;30(7):683–695. doi: 10.1111/j.1348-0421.1986.tb02994.x. [DOI] [PubMed] [Google Scholar]
  10. Landini M. P., Re M. C., Mirolo G., Baldassarri B., La Placa M. Human immune response to cytomegalovirus structural polypeptides studied by immunoblotting. J Med Virol. 1985 Dec;17(4):303–311. doi: 10.1002/jmv.1890170403. [DOI] [PubMed] [Google Scholar]
  11. Landini M. P., Ripalti A. A DNA-nicking activity associated with the nucleocapsid of human cytomegalovirus. Arch Virol. 1982;73(3-4):351–356. doi: 10.1007/BF01318089. [DOI] [PubMed] [Google Scholar]
  12. Landini M. P., Rossier E., Schmitz H. Antibodies to human cytomegalovirus structural polypeptides during primary infection. J Virol Methods. 1988 Dec;22(2-3):309–317. doi: 10.1016/0166-0934(88)90113-9. [DOI] [PubMed] [Google Scholar]
  13. Mocarski E. S., Pereira L., Michael N. Precise localization of genes on large animal virus genomes: use of lambda gt11 and monoclonal antibodies to map the gene for a cytomegalovirus protein family. Proc Natl Acad Sci U S A. 1985 Feb;82(4):1266–1270. doi: 10.1073/pnas.82.4.1266. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pass R. F., Griffiths P. D., August A. M. Antibody response to cytomegalovirus after renal transplantation: comparison of patients with primary and recurrent infections. J Infect Dis. 1983 Jan;147(1):40–46. doi: 10.1093/infdis/147.1.40. [DOI] [PubMed] [Google Scholar]
  15. Rasmussen L., Kelsall D., Nelson R., Carney W., Hirsch M., Winston D., Preiksaitis J., Merigan T. C. Virus-specific IgG and IgM antibodies in normal and immunocompromised subjects infected with cytomegalovirus. J Infect Dis. 1982 Feb;145(2):191–199. doi: 10.1093/infdis/145.2.191. [DOI] [PubMed] [Google Scholar]
  16. Revello M. G., Percivalle E., Gerna G. Immunoglobulin M to the membrane of uninfected fibroblasts in primary human cytomegalovirus infections. Microbiologica. 1986 Apr;9(2):127–138. [PubMed] [Google Scholar]
  17. Ripalti A., Landini M. P., Mocarski E. S., La Placa M. Identification and preliminary use of recombinant lambda gt11 fusion proteins in human cytomegalovirus diagnosis. J Gen Virol. 1989 May;70(Pt 5):1247–1251. doi: 10.1099/0022-1317-70-5-1247. [DOI] [PubMed] [Google Scholar]
  18. Schmitz H., Mohr H., Majoubi M. Differentiation between primary and recurrent cytomegalovirus infections. Arch Virol. 1986;90(1-2):41–51. doi: 10.1007/BF01314143. [DOI] [PubMed] [Google Scholar]
  19. Scholl B. C., Von Hintzenstern J., Borisch B., Traupe B., Bröker M., Jahn G. Prokaryotic expression of immunogenic polypeptides of the large phosphoprotein (pp150) of human cytomegalovirus. J Gen Virol. 1988 Jun;69(Pt 6):1195–1204. doi: 10.1099/0022-1317-69-6-1195. [DOI] [PubMed] [Google Scholar]
  20. Stern H., Tucker S. M. Prospective study of cytomegalovirus infection in pregnancy. Br Med J. 1973 May 5;2(5861):268–270. doi: 10.1136/bmj.2.5861.268. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES