Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1989 Oct;27(10):2369–2372. doi: 10.1128/jcm.27.10.2369-2372.1989

DNA probe for the identification of Histoplasma capsulatum.

E J Keath 1, E D Spitzer 1, A A Painter 1, S J Travis 1, G S Kobayashi 1, G Medoff 1
PMCID: PMC267026  PMID: 2573616

Abstract

A 1.85-kilobase HindIII nuclear DNA probe from Histoplasma capsulatum G217B detected polymorphic restriction fragments within whole-cell DNA from different clinical isolates of H. capsulatum, consistent with the previous system of classification. The probe failed to hybridize to DNA from Blastomyces dermatitidis, Candida spp., Saccharomyces cerevisiae, Sepedonium chrysospermum, and Chrysosporium keratinophilum under low-stringency conditions and therefore may have value as a diagnostic reagent to identify H. capsulatum.

Full text

PDF
2369

Images in this article

2370Image
on p.2370
2371Image
on p.2371
2371Image
on p.2371

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akins R. A., Lambowitz A. M. General method for cloning Neurospora crassa nuclear genes by complementation of mutants. Mol Cell Biol. 1985 Sep;5(9):2272–2278. doi: 10.1128/mcb.5.9.2272. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carle G. F., Olson M. V. An electrophoretic karyotype for yeast. Proc Natl Acad Sci U S A. 1985 Jun;82(11):3756–3760. doi: 10.1073/pnas.82.11.3756. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Farr C. J., Saiki R. K., Erlich H. A., McCormick F., Marshall C. J. Analysis of RAS gene mutations in acute myeloid leukemia by polymerase chain reaction and oligonucleotide probes. Proc Natl Acad Sci U S A. 1988 Mar;85(5):1629–1633. doi: 10.1073/pnas.85.5.1629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Keath E. J., Painter A. A., Kobayashi G. S., Medoff G. Variable expression of a yeast-phase-specific gene in Histoplasma capsulatum strains differing in thermotolerance and virulence. Infect Immun. 1989 May;57(5):1384–1390. doi: 10.1128/iai.57.5.1384-1390.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Medoff G., Maresca B., Lambowitz A. M., Kobayashi G., Painter A., Sacco M., Carratu L. Correlation between pathogenicity and temperature sensitivity in different strains of Histoplasma capsulatum. J Clin Invest. 1986 Dec;78(6):1638–1647. doi: 10.1172/JCI112757. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Pace N. R., Olsen G. J., Woese C. R. Ribosomal RNA phylogeny and the primary lines of evolutionary descent. Cell. 1986 May 9;45(3):325–326. doi: 10.1016/0092-8674(86)90315-6. [DOI] [PubMed] [Google Scholar]
  7. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  8. Riggsby W. S., Torres-Bauza L. J., Wills J. W., Townes T. M. DNA content, kinetic complexity, and the ploidy question in Candida albicans. Mol Cell Biol. 1982 Jul;2(7):853–862. doi: 10.1128/mcb.2.7.853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Saiki R. K., Bugawan T. L., Horn G. T., Mullis K. B., Erlich H. A. Analysis of enzymatically amplified beta-globin and HLA-DQ alpha DNA with allele-specific oligonucleotide probes. Nature. 1986 Nov 13;324(6093):163–166. doi: 10.1038/324163a0. [DOI] [PubMed] [Google Scholar]
  10. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  11. Spitzer E. D., Lasker B. A., Travis S. J., Kobayashi G. S., Medoff G. Use of mitochondrial and ribosomal DNA polymorphisms to classify clinical and soil isolates of Histoplasma capsulatum. Infect Immun. 1989 May;57(5):1409–1412. doi: 10.1128/iai.57.5.1409-1412.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Standard P. G., Kaufman L. Specific immunological test for the rapid identification of members of the genus Histoplasma. J Clin Microbiol. 1976 Feb;3(2):191–199. doi: 10.1128/jcm.3.2.191-199.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Vincent R. D., Goewert R., Goldman W. E., Kobayashi G. S., Lambowitz A. M., Medoff G. Classification of Histoplasma capsulatum isolates by restriction fragment polymorphisms. J Bacteriol. 1986 Mar;165(3):813–818. doi: 10.1128/jb.165.3.813-818.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wellems T. E., Walliker D., Smith C. L., do Rosario V. E., Maloy W. L., Howard R. J., Carter R., McCutchan T. F. A histidine-rich protein gene marks a linkage group favored strongly in a genetic cross of Plasmodium falciparum. Cell. 1987 Jun 5;49(5):633–642. doi: 10.1016/0092-8674(87)90539-3. [DOI] [PubMed] [Google Scholar]
  15. Williams J. E., Moser S. A. Chronic murine pulmonary blastomycosis induced by intratracheally inoculated Blastomyces dermatitidis conidia. Am Rev Respir Dis. 1987 Jan;135(1):17–25. doi: 10.1164/arrd.1987.135.1.17. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES