Abstract
A passive hemagglutination test (PHA) for detecting human immunodeficiency virus type 1 antibodies in serum samples by using envelope glycoprotein (gp160)-coupled sheep erythrocytes was described earlier (M.B. Vasudevachari, K. U. Uffelman, T.C. Mast, R.L. Dewar, V. Natarajan, H.C. Lane, and N.P. Salzman, J. Clin. Microbiol. 27:179-181, 1989). In the study reported here, the applicability of the PHA test to the detection of antibodies in whole-blood and saliva samples has been investigated. We observed a 100% correlation between PHA and commercial enzyme-linked immunosorbent assay in 101 whole-blood samples and 98% correlation between PHA and reactivity to envelope proteins in Western blots (immunoblots) of 53 saliva samples. Furthermore, salivary antibodies could be detected in 19 of the 22 seropositive individuals. As in serum, antibodies to envelope proteins were widely prevalent in all the Western blot-reactive saliva samples.
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- Archibald D. W., Barr C. E., Torosian J. P., McLane M. F., Essex M. Secretory IgA antibodies to human immunodeficiency virus in the parotid saliva of patients with AIDS and AIDS-related complex. J Infect Dis. 1987 Apr;155(4):793–796. doi: 10.1093/infdis/155.4.793. [DOI] [PubMed] [Google Scholar]
- Archibald D. W., Zon L. I., Groopman J. E., Allan J. S., McLane M. F., Essex M. E. Salivary antibodies as a means of detecting human T cell lymphotropic virus type III/lymphadenopathy-associated virus infection. J Clin Microbiol. 1986 Nov;24(5):873–875. doi: 10.1128/jcm.24.5.873-875.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Archibald D. W., Zon L., Groopman J. E., McLane M. F., Essex M. Antibodies to human T-lymphotropic virus type III (HTLV-III) in saliva of acquired immunodeficiency syndrome (AIDS) patients and in persons at risk for AIDS. Blood. 1986 Mar;67(3):831–834. [PubMed] [Google Scholar]
- Kemp B. E., Rylatt D. B., Bundesen P. G., Doherty R. R., McPhee D. A., Stapleton D., Cottis L. E., Wilson K., John M. A., Khan J. M. Autologous red cell agglutination assay for HIV-1 antibodies: simplified test with whole blood. Science. 1988 Sep 9;241(4871):1352–1354. doi: 10.1126/science.3413497. [DOI] [PubMed] [Google Scholar]
- Parry J. V., Perry K. R., Mortimer P. P. Sensitive assays for viral antibodies in saliva: an alternative to tests on serum. Lancet. 1987 Jul 11;2(8550):72–75. doi: 10.1016/s0140-6736(87)92737-1. [DOI] [PubMed] [Google Scholar]
- Vasudevachari M. B., Uffelman K. W., Mast T. C., Dewar R. L., Natarajan V., Lane H. C., Salzman N. P. Passive hemagglutination test for detection of antibodies to human immunodeficiency virus type 1 and comparison of the test with enzyme-linked immunosorbent assay and Western blot (immunoblot) analysis. J Clin Microbiol. 1989 Jan;27(1):179–181. doi: 10.1128/jcm.27.1.179-181.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]