Abstract
GC-Lect, a new selective medium for the isolation of Neisseria gonorrhoeae which contains five antimicrobial agents, was evaluated with stock cultures and with 500 clinical specimens. With stock cultures, vancomycin-resistant Staphylococcus epidermidis that grew on modified Thayer-Martin medium (MTM) was inhibited on the new medium. Also, vancomycin-susceptible strains of N. gonorrhoeae were much less inhibited on the new medium than on Martin-Lewis agar or MTM. With oropharyngeal cultures of healthy volunteers, Capnocytophaga species were frequently isolated on MTM from two of three manufacturers but were completely inhibited on GC-Lect. In the clinical study, visible growth of N. gonorrhoeae occurred within 24 h in 72% of the positive cultures on GC-Lect, compared with only 52% on the reference medium. A total of 50 positive cultures were obtained with GC-Lect, compared with 49 obtained with MTM. The selectivity of GC-Lect was superior, with only 19 cultures producing growth of normal flora, compared with 78 cultures on MTM after 24 h of incubation. The selectivity was especially improved on GC-Lect with regard to yeasts (2 versus 30 cultures) and gram-positive cocci (5 versus 31 cultures).
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- Cross R. C., Hoger M. B., Neibaur R., Pasternack B., Brady F. J. VCN--inhibited strains of Neisseria gonorrhoeae. HSMHA Health Rep. 1971 Nov;86(11):990–992. [PMC free article] [PubMed] [Google Scholar]
- Faur Y. C., Weisburd M. H., Wilson M. E. The selectivity of vancomycin and lincomycin in NYC medium for the recovery of N. gonorrhoeae from clinical specimens. Health Lab Sci. 1978 Jan;15(1):22–27. [PubMed] [Google Scholar]
- Miller M. A., Anderson P., Parker J. W., Rohrer H. H. Inhibition of Neisseria gonorrhoeae isolates by Martin-Lewis medium. Epidemiology, susceptibility profile, and plasma analysis. Br J Vener Dis. 1982 Apr;58(2):96–100. doi: 10.1136/sti.58.2.96. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mirrett S., Reller L. B., Knapp J. S. Neisseria gonorrhoeae strains inhibited by vancomycin in selective media and correlation with auxotype. J Clin Microbiol. 1981 Jul;14(1):94–99. doi: 10.1128/jcm.14.1.94-99.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Phillips I., Humphrey D., Middleton A., Nicol C. S. Diagnosis of gonorrhoea by culture on a selective medium containing vancomycin, colistin, nystatin and trimethoprim (VCNT). A comparison with Gram-staining and immunofluorescence. Br J Vener Dis. 1972 Aug;48(4):287–292. doi: 10.1136/sti.48.4.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reichart C. A., Rupkey L. M., Brady W. E., Hook E. W., 3rd Comparison of GC-Lect and modified Thayer-Martin media for isolation of Neisseria gonorrhoeae. J Clin Microbiol. 1989 May;27(5):808–811. doi: 10.1128/jcm.27.5.808-811.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reyn A., Bentzon M. W. Comparison of a selective and a non-selective medium in the diagnosis of gonorrhoea to ascertain the sensitivity of Neisseria gonorrhoeae to vancomycin. Br J Vener Dis. 1972 Oct;48(5):363–368. doi: 10.1136/sti.48.5.363. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Windall J. J., Hall M. M., Washington J. A., 2nd, Douglass T. J., Weed L. A. Inhibitory effects of vancomycin on Neisseria gonorrhoeae in Thayer-Martin medium. J Infect Dis. 1980 Nov;142(5):775–775. doi: 10.1093/infdis/142.5.775. [DOI] [PubMed] [Google Scholar]