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. 2009 Feb 19;58(5):1067–1076. doi: 10.2337/db08-1233

FIG. 5.

FIG. 5.

Improved glucose tolerance in hGPR40 transgenic mice harboring diabetic KK background on regular diet. A: Human and mouse GPR40 mRNA levels in islets from hGPR40 transgenic × KK and nontransgenic × KK mice (n = 3) by quantitative real-time PCR analyses. mRNA levels of actin were used as an internal control. B–E: Oral glucose tolerance test for hGPR40 transgenic mice and nontransgenic mice harboring hybrid background. Glucose was administered orally at 2 g/kg body wt. B and C: Plasma glucose (B) and plasma insulin (C) at 12 weeks of age on regular diet. Data in panel D represent the AUC0–120 min of plasma glucose shown in panel B, and data in panel E represent the AUC0–30 min of plasma insulin shown in panel C. F and G: Glucose- and palmitate-stimulated insulin secretion in isolated islets from hGPR40 transgenic × KK and nontransgenic × KK mice. Islets were isolated from regular diet–fed mice at 12 weeks. Five islets with similar sizes from each group (four batches in each group) were used. All values are means ± SE (n = 8–10). **P ≤ 0.01, *P ≤ 0.05 vs. nontransgenic mice by Student's t test. NonTg, nontransgenic; Tg, transgenic.