Abstract
A latex agglutination assay for the detection of protein A-secreting Staphylococcus aureus strains or strains with protein A in the cell wall is described. The assay utilizes latex particles coated with chicken anti-protein A antibodies. Chicken antibodies do not react with protein G-producing streptococci or rheumatoid factor, thus avoiding false-positive reactions.
Full text
PDF

Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Björck L., Kronvall G. Purification and some properties of streptococcal protein G, a novel IgG-binding reagent. J Immunol. 1984 Aug;133(2):969–974. [PubMed] [Google Scholar]
- Essers L., Radebold K. Rapid and reliable identification of Staphylococcus aureus by a latex agglutination test. J Clin Microbiol. 1980 Nov;12(5):641–643. doi: 10.1128/jcm.12.5.641-643.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Forsgren A. Significance of protein a production by staphylococci. Infect Immun. 1970 Nov;2(5):672–673. doi: 10.1128/iai.2.5.672-673.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hjelm H., Hjelm K., Sjöquist J. Protein A from Staphylococcus aureus. Its isolation by affinity chromatography and its use as an immunosorbent for isolation of immunoglobulins. FEBS Lett. 1972 Nov 15;28(1):73–76. doi: 10.1016/0014-5793(72)80680-x. [DOI] [PubMed] [Google Scholar]
- Hädge D., Ambrosius H. Evolution of low molecular weight immunoglobulins--IV. IgY-like immunoglobulins of birds, reptiles and amphibians, precursors of mammalian IgA. Mol Immunol. 1984 Aug;21(8):699–707. doi: 10.1016/0161-5890(84)90022-1. [DOI] [PubMed] [Google Scholar]
- Jensenius J. C., Andersen I., Hau J., Crone M., Koch C. Eggs: conveniently packaged antibodies. Methods for purification of yolk IgG. J Immunol Methods. 1981;46(1):63–68. doi: 10.1016/0022-1759(81)90333-1. [DOI] [PubMed] [Google Scholar]
- Larsson A., Sjöquist J. Chicken antibodies: a tool to avoid false positive results by rheumatoid factor in latex fixation tests. J Immunol Methods. 1988 Apr 6;108(1-2):205–208. doi: 10.1016/0022-1759(88)90420-6. [DOI] [PubMed] [Google Scholar]
- Lindmark R., Movitz J., Sjöquist J. Extracellular protein A from a methicillin-resistant strain of Staphylococcus aureus. Eur J Biochem. 1977 Apr 15;74(3):623–628. doi: 10.1111/j.1432-1033.1977.tb11431.x. [DOI] [PubMed] [Google Scholar]
- Lindmark R., Thorén-Tolling K., Sjöquist J. Binding of immunoglobulins to protein A and immunoglobulin levels in mammalian sera. J Immunol Methods. 1983 Aug 12;62(1):1–13. doi: 10.1016/0022-1759(83)90104-7. [DOI] [PubMed] [Google Scholar]
- Sjöquist J., Movitz J., Johansson I. B., Hjelm H. Localization of protein A in the bacteria. Eur J Biochem. 1972 Oct 17;30(1):190–194. doi: 10.1111/j.1432-1033.1972.tb02086.x. [DOI] [PubMed] [Google Scholar]
- Sperber W. H. The identification of staphylococci in clinical and food microbiology laboratories. CRC Crit Rev Clin Lab Sci. 1976;7(2):121–184. doi: 10.3109/10408367709151577. [DOI] [PubMed] [Google Scholar]
