Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1989 Dec;27(12):2856–2857. doi: 10.1128/jcm.27.12.2856-2857.1989

Novel latex agglutination method with chicken anti-protein A for detection of Staphylococcus aureus infections.

A Larsson 1, J Sjöquist 1
PMCID: PMC267143  PMID: 2592547

Abstract

A latex agglutination assay for the detection of protein A-secreting Staphylococcus aureus strains or strains with protein A in the cell wall is described. The assay utilizes latex particles coated with chicken anti-protein A antibodies. Chicken antibodies do not react with protein G-producing streptococci or rheumatoid factor, thus avoiding false-positive reactions.

Full text

PDF
2856

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Björck L., Kronvall G. Purification and some properties of streptococcal protein G, a novel IgG-binding reagent. J Immunol. 1984 Aug;133(2):969–974. [PubMed] [Google Scholar]
  2. Essers L., Radebold K. Rapid and reliable identification of Staphylococcus aureus by a latex agglutination test. J Clin Microbiol. 1980 Nov;12(5):641–643. doi: 10.1128/jcm.12.5.641-643.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Forsgren A. Significance of protein a production by staphylococci. Infect Immun. 1970 Nov;2(5):672–673. doi: 10.1128/iai.2.5.672-673.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hjelm H., Hjelm K., Sjöquist J. Protein A from Staphylococcus aureus. Its isolation by affinity chromatography and its use as an immunosorbent for isolation of immunoglobulins. FEBS Lett. 1972 Nov 15;28(1):73–76. doi: 10.1016/0014-5793(72)80680-x. [DOI] [PubMed] [Google Scholar]
  5. Hädge D., Ambrosius H. Evolution of low molecular weight immunoglobulins--IV. IgY-like immunoglobulins of birds, reptiles and amphibians, precursors of mammalian IgA. Mol Immunol. 1984 Aug;21(8):699–707. doi: 10.1016/0161-5890(84)90022-1. [DOI] [PubMed] [Google Scholar]
  6. Jensenius J. C., Andersen I., Hau J., Crone M., Koch C. Eggs: conveniently packaged antibodies. Methods for purification of yolk IgG. J Immunol Methods. 1981;46(1):63–68. doi: 10.1016/0022-1759(81)90333-1. [DOI] [PubMed] [Google Scholar]
  7. Larsson A., Sjöquist J. Chicken antibodies: a tool to avoid false positive results by rheumatoid factor in latex fixation tests. J Immunol Methods. 1988 Apr 6;108(1-2):205–208. doi: 10.1016/0022-1759(88)90420-6. [DOI] [PubMed] [Google Scholar]
  8. Lindmark R., Movitz J., Sjöquist J. Extracellular protein A from a methicillin-resistant strain of Staphylococcus aureus. Eur J Biochem. 1977 Apr 15;74(3):623–628. doi: 10.1111/j.1432-1033.1977.tb11431.x. [DOI] [PubMed] [Google Scholar]
  9. Lindmark R., Thorén-Tolling K., Sjöquist J. Binding of immunoglobulins to protein A and immunoglobulin levels in mammalian sera. J Immunol Methods. 1983 Aug 12;62(1):1–13. doi: 10.1016/0022-1759(83)90104-7. [DOI] [PubMed] [Google Scholar]
  10. Sjöquist J., Movitz J., Johansson I. B., Hjelm H. Localization of protein A in the bacteria. Eur J Biochem. 1972 Oct 17;30(1):190–194. doi: 10.1111/j.1432-1033.1972.tb02086.x. [DOI] [PubMed] [Google Scholar]
  11. Sperber W. H. The identification of staphylococci in clinical and food microbiology laboratories. CRC Crit Rev Clin Lab Sci. 1976;7(2):121–184. doi: 10.3109/10408367709151577. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES