Abstract
Nineteen isolates of Mycobacterium haemophilum were analyzed by pulsed-field gel electrophoresis of large restriction fragments generated by digestion of chromosomal DNA with XbaI. Six patterns were observed. Twelve of 16 M. haemophilum isolates (75%) collected in the New York Metropolitan Area from 1990 to 1991 shared the same pattern, including all six isolates submitted from one hospital. Two different patterns were seen among the other four isolates. Individual isolates from Albany, N.Y., Florida, and Texas had unique patterns. Pulsed-field gel electrophoresis is the first method reported with the capability to type strains of M. haemophilum and will hopefully provide insight into the source and transmission of this emerging pathogen.
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- Becherer P., Hopfer R. L. Infection with Mycobacterium haemophilum. Clin Infect Dis. 1992 Mar;14(3):793–793. doi: 10.1093/clinids/14.3.793. [DOI] [PubMed] [Google Scholar]
- Butler W. R., Jost K. C., Jr, Kilburn J. O. Identification of mycobacteria by high-performance liquid chromatography. J Clin Microbiol. 1991 Nov;29(11):2468–2472. doi: 10.1128/jcm.29.11.2468-2472.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Collins C. H., Grange J. M., Yates M. D. Mycobacteria in water. J Appl Bacteriol. 1984 Oct;57(2):193–211. doi: 10.1111/j.1365-2672.1984.tb01384.x. [DOI] [PubMed] [Google Scholar]
- Davis B. R., Brumbach J., Sanders W. J., Wolinsky E. Skin lesions caused by Mycobacterium haemophilum. Ann Intern Med. 1982 Nov;97(5):723–724. doi: 10.7326/0003-4819-97-5-723. [DOI] [PubMed] [Google Scholar]
- Gouby A., Branger B., Oules R., Ramuz M. Two cases of Mycobacterium haemophilum infection in a renal-dialysis unit. J Med Microbiol. 1988 Apr;25(4):299–300. doi: 10.1099/00222615-25-4-299. [DOI] [PubMed] [Google Scholar]
- Kristjansson M., Bieluch V. M., Byeff P. D. Mycobacterium haemophilum infection in immunocompromised patients: case report and review of the literature. Rev Infect Dis. 1991 Sep-Oct;13(5):906–910. doi: 10.1093/clinids/13.5.906. [DOI] [PubMed] [Google Scholar]
- Portaels F., Dawson D. J., Larsson L., Rigouts L. Biochemical properties and fatty acid composition of Mycobacterium haemophilum: study of 16 isolates from Australian patients. J Clin Microbiol. 1993 Jan;31(1):26–30. doi: 10.1128/jcm.31.1.26-30.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zhang Y., Mazurek G. H., Cave M. D., Eisenach K. D., Pang Y., Murphy D. T., Wallace R. J., Jr DNA polymorphisms in strains of Mycobacterium tuberculosis analyzed by pulsed-field gel electrophoresis: a tool for epidemiology. J Clin Microbiol. 1992 Jun;30(6):1551–1556. doi: 10.1128/jcm.30.6.1551-1556.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]