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. 2008 Dec 23;150(5):2395–2403. doi: 10.1210/en.2008-1027

Figure 3.

Figure 3

Mutation of Sp1, SF-1, and Egr-1 binding elements on the LHβ promoter does not alleviate progesterone suppression of LHβ transcription. A, A schematic of the rat LHβ promoter illustrating the known transcription factor binding sites involved in basal and GnRH-induction of LHβ gene expression. The rat LHβ promoter contains two Sp1 sites at −451/−442 and −398/−386. The most distal Sp1 element overlaps with a CArG box (−443/−434). The proximal promoter includes two SF-1 binding elements at −127/−119 and −58/−51 and two Egr-1 binding sites at −112/−104 and −49/−41. B and C, LβT2 cells were transiently transfected with either the −1800-bp LHβ-luc reporter plasmid or reporter plasmids containing mutations in the Sp1, SF-1, or Egr-1 binding elements as indicated. After overnight starvation in serum-free media, the cells were treated with vehicle, 100 nm R5020, 10 nm GnRH, or both hormones as indicated. *, Significant differences from the vehicle-treated control; †, interaction as defined by a two-way ANOVA (P < 0.05). luc, Luciferase.