Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1994 Apr;32(4):1119–1120. doi: 10.1128/jcm.32.4.1119-1120.1994

Effect of delayed processing of blood samples on performance of cytomegalovirus antigenemia assay.

J Niubò 1, J L Pérez 1, A Carvajal 1, C Ardanuy 1, R Martín 1
PMCID: PMC267202  PMID: 8027328

Abstract

This prospective, parallel, and blind study of 120 blood samples from immunocompromised patients examined the influence of delayed sample processing on cytomegalovirus antigenemia assay. Cytomegalovirus was detected in 49 samples (40.8%): 41 were processed within 2 to 4 h, and 40 were reprocessed the following day. Results were discrepant in 17 of the samples with the lowest positive cell counts. Differences between the two days did not reach statistical significance.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bein G., Bitsch A., Hoyer J., Kirchner H. The detection of human cytomegalovirus immediate early antigen in peripheral blood leucocytes. J Immunol Methods. 1991 Mar 21;137(2):175–180. doi: 10.1016/0022-1759(91)90022-8. [DOI] [PubMed] [Google Scholar]
  2. Erice A., Holm M. A., Gill P. C., Henry S., Dirksen C. L., Dunn D. L., Hillam R. P., Balfour H. H., Jr Cytomegalovirus (CMV) antigenemia assay is more sensitive than shell vial cultures for rapid detection of CMV in polymorphonuclear blood leukocytes. J Clin Microbiol. 1992 Nov;30(11):2822–2825. doi: 10.1128/jcm.30.11.2822-2825.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gerna G., Revello M. G., Percivalle E., Morini F. Comparison of different immunostaining techniques and monoclonal antibodies to the lower matrix phosphoprotein (pp65) for optimal quantitation of human cytomegalovirus antigenemia. J Clin Microbiol. 1992 May;30(5):1232–1237. doi: 10.1128/jcm.30.5.1232-1237.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gleaves C. A., Smith T. F., Shuster E. A., Pearson G. R. Comparison of standard tube and shell vial cell culture techniques for the detection of cytomegalovirus in clinical specimens. J Clin Microbiol. 1985 Feb;21(2):217–221. doi: 10.1128/jcm.21.2.217-221.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Michalski F. J. Specimens for cytomegalovirus antigenemia assay. J Clin Microbiol. 1993 May;31(5):1400–1401. doi: 10.1128/jcm.31.5.1400-1401.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Miller H., Rossier E., Milk R., Thomas C. Prospective study of cytomegalovirus antigenemia in allograft recipients. J Clin Microbiol. 1991 May;29(5):1054–1055. doi: 10.1128/jcm.29.5.1054-1055.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Revello M. G., Percivalle E., Zavattoni M., Parea M., Grossi P., Gerna G. Detection of human cytomegalovirus immediate early antigen in leukocytes as a marker of viremia in immunocompromised patients. J Med Virol. 1989 Oct;29(2):88–93. doi: 10.1002/jmv.1890290204. [DOI] [PubMed] [Google Scholar]
  8. The T. H., van der Bij W., van den Berg A. P., van der Giessen M., Weits J., Sprenger H. G., van Son W. J. Cytomegalovirus antigenemia. Rev Infect Dis. 1990 Sep-Oct;12 (Suppl 7):S734–S744. [PubMed] [Google Scholar]
  9. The T. H., van der Ploeg M., van den Berg A. P., Vlieger A. M., van der Giessen M., van Son W. J. Direct detection of cytomegalovirus in peripheral blood leukocytes--a review of the antigenemia assay and polymerase chain reaction. Transplantation. 1992 Aug;54(2):193–198. doi: 10.1097/00007890-199208000-00001. [DOI] [PubMed] [Google Scholar]
  10. van der Bij W., Schirm J., Torensma R., van Son W. J., Tegzess A. M., The T. H. Comparison between viremia and antigenemia for detection of cytomegalovirus in blood. J Clin Microbiol. 1988 Dec;26(12):2531–2535. doi: 10.1128/jcm.26.12.2531-2535.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. van der Giessen M., The T. H., van Son W. J. Cytomegalovirus antigenemia assay. J Clin Microbiol. 1991 Dec;29(12):2909–2910. doi: 10.1128/jcm.29.12.2909-2910.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES