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Fourth order polynomial curves were fitted to the background subtracted GC frequency curves of promoter sets (associated or not with CpG islands) between – 1000 to – 40 bp relative to TSS. R2 values for the fitted curves to promoters associated or not associated with CpG islands are 0.9985 and 0.9668 in humans and 0.9979 and 9844 in mouse respectively.
