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. 1989 Jan;27(1):199–200. doi: 10.1128/jcm.27.1.199-200.1989

Detection of herpes simplex virus by 8 h in shell vial cultures with primary rabbit kidney cells.

P Tse 1, S L Aarnaes 1, L M de la Maza 1, E M Peterson 1
PMCID: PMC267264  PMID: 2536389

Abstract

Shell vial (SV) cultures for herpes simplex virus using primary rabbit kidney cells stained at 8 h after inoculation were compared with 20-h SV cultures as well as conventional tissue culture. Of the 326 clinical specimens examined, conventional culture detected 67, and of these, 61 (91%) and 42 (63%) were detected by 20-h SV cultures and 8-h SV cultures, respectively.

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Selected References

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  1. Darougar S., Walpita P., Thaker U., Goh B. T., Dunlop E. M. A rapid and sensitive culture test for the laboratory diagnosis of genital herpes in women. Genitourin Med. 1986 Apr;62(2):93–96. doi: 10.1136/sti.62.2.93. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fayram S. L., Aarnaes S. L., Peterson E. M., de la Maza L. M. Evaluation of five cell types for the isolation of herpes simplex virus. Diagn Microbiol Infect Dis. 1986 Jul;5(2):127–133. doi: 10.1016/0732-8893(86)90114-8. [DOI] [PubMed] [Google Scholar]
  3. Gleaves C. A., Wilson D. J., Wold A. D., Smith T. F. Detection and serotyping of herpes simplex virus in MRC-5 cells by use of centrifugation and monoclonal antibodies 16 h postinoculation. J Clin Microbiol. 1985 Jan;21(1):29–32. doi: 10.1128/jcm.21.1.29-32.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Halstead D. C., Beckwith D. G., Sautter R. L., Plosila L., Schneck K. A. Evaluation of a rapid latex slide agglutination test for herpes simplex virus as a specimen screen and culture identification method. J Clin Microbiol. 1987 May;25(5):936–937. doi: 10.1128/jcm.25.5.936-937.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lafferty W. E., Krofft S., Remington M., Giddings R., Winter C., Cent A., Corey L. Diagnosis of herpes simplex virus by direct immunofluorescence and viral isolation from samples of external genital lesions in a high-prevalence population. J Clin Microbiol. 1987 Feb;25(2):323–326. doi: 10.1128/jcm.25.2.323-326.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Land S. A., Skurrie I. J., Gilbert G. L. Rapid diagnosis of herpes simplex virus infections by enzyme-linked immunosorbent assay. J Clin Microbiol. 1984 Jun;19(6):865–869. doi: 10.1128/jcm.19.6.865-869.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Langenberg A., Smith D., Brakel C. L., Pollice M., Remington M., Winter C., Dunne A., Corey L. Detection of herpes simplex virus DNA from genital lesions by in situ hybridization. J Clin Microbiol. 1988 May;26(5):933–937. doi: 10.1128/jcm.26.5.933-937.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MacDonald R. L., Hughes B. L., Aarnaes S. L., Peterson E. M., de la Maza L. M. Evaluation of a shell vial centrifugation method for the detection of herpes simplex virus. Diagn Microbiol Infect Dis. 1988 Jan;9(1):51–54. doi: 10.1016/0732-8893(88)90061-2. [DOI] [PubMed] [Google Scholar]
  9. Peterson E. M., Hughes B. L., Aarnaes S. L., de la Maza L. M. Comparison of primary rabbit kidney and MRC-5 cells and two stain procedures for herpes simplex virus detection by a shell vial centrifugation method. J Clin Microbiol. 1988 Feb;26(2):222–224. doi: 10.1128/jcm.26.2.222-224.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Pruneda R. C., Almanza I. Centrifugation-shell vial technique for rapid detection of herpes simplex virus cytopathic effect in Vero cells. J Clin Microbiol. 1987 Feb;25(2):423–424. doi: 10.1128/jcm.25.2.423-424.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Salmon V. C., Turner R. B., Speranza M. J., Overall J. C., Jr Rapid detection of herpes simplex virus in clinical specimens by centrifugation and immunoperoxidase staining. J Clin Microbiol. 1986 Apr;23(4):683–686. doi: 10.1128/jcm.23.4.683-686.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Zhao L. S., Landry M. L., Balkovic E. S., Hsiung G. D. Impact of cell culture sensitivity and virus concentration on rapid detection of herpes simplex virus by cytopathic effects and immunoperoxidase staining. J Clin Microbiol. 1987 Aug;25(8):1401–1405. doi: 10.1128/jcm.25.8.1401-1405.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

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