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. 1989 Jan;27(1):223–224. doi: 10.1128/jcm.27.1.223-224.1989

Comparison of three methods for detection of Cryptosporidium oocysts in a low-prevalence population.

E J Baron 1, C Schenone 1, B Tanenbaum 1
PMCID: PMC267273  PMID: 2643623

Abstract

Four hundred fecal specimens which had been received for routine ova and parasite examination were concentrated by Formalin-ether sedimentation. Sediments were examined as saline and iodine-stained wet preparations and were stained with rhodamine-auramine O and a commercially available monoclonal fluorescent-antibody stain for oocysts of Cryptosporidium species. Examination with the fluorescent stains detected cryptosporidia in both positive specimens (0.5% prevalence), and routine direct wet-preparation examination detected cryptosporidia in one of them. Detection of only low numbers of positive specimens in our nonrisk population argues against routine use of specific and expensive stain reagents.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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