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. 1989 Feb;27(2):346–347. doi: 10.1128/jcm.27.2.346-347.1989

Growth of Aeromonas spp. on Butzler Campylobacter selective agar and evaluation of the agar for the primary isolation of Aeromonas spp. from clinical specimens.

S K Misra 1, R K Bhadra 1, S C Pal 1, G B Nair 1
PMCID: PMC267308  PMID: 2915030

Abstract

The fortuitous finding that Aeromonas spp. grew well on Butzler Campylobacter selective medium (BCSA) in a microaerobic atmosphere at 42 degrees C prompted us to evaluate the performance of BCSA for selective isolation of aeromonads in comparison with ampicillin (30 micrograms/ml) sheep blood agar (ASBA30). Overall recovery rates of aeromonads from 563 stool samples from patients with acute diarrhea were higher on ASBA30 (70.4%) than on BCSA (56.3%); however, 21 (29.5%) grew only on BCSA. The three human-associated Aeromonas spp. could be recovered on BCSA and ASBA30. We recommend the use of BCSA to laboratories reluctant to include a specific selective medium for aeromonads.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Butzler J. P., Dekeyser P., Detrain M., Dehaen F. Related vibrio in stools. J Pediatr. 1973 Mar;82(3):493–495. doi: 10.1016/s0022-3476(73)80131-3. [DOI] [PubMed] [Google Scholar]
  2. Dekeyser P., Gossuin-Detrain M., Butzler J. P., Sternon J. Acute enteritis due to related vibrio: first positive stool cultures. J Infect Dis. 1972 Apr;125(4):390–392. doi: 10.1093/infdis/125.4.390. [DOI] [PubMed] [Google Scholar]
  3. Kaper J., Seidler R. J., Lockman H., Colwell R. R. Medium for the presumptive identification of Aeromonas hydrophila and Enterobacteriaceae. Appl Environ Microbiol. 1979 Nov;38(5):1023–1026. doi: 10.1128/aem.38.5.1023-1026.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kay B. A., Guerrero C. E., Sack R. B. Media for the isolation of Aeromonas hydrophila. J Clin Microbiol. 1985 Nov;22(5):888–890. doi: 10.1128/jcm.22.5.888-890.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Millership S. E., Chattopadhyay B. Methods for the isolation of Aeromonas hydrophila and Plesiomonas shigelloides from faeces. J Hyg (Lond) 1984 Apr;92(2):145–152. doi: 10.1017/s0022172400064159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mishra S., Nair G. B., Bhadra R. K., Sikder S. N., Pal S. C. Comparison of selective media for primary isolation of Aeromonas species from human and animal feces. J Clin Microbiol. 1987 Nov;25(11):2040–2043. doi: 10.1128/jcm.25.11.2040-2043.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Moskowitz L. B., Chester B. Growth of non-Campylobacter, oxidase-positive bacteria on selective Campylobacter agar. J Clin Microbiol. 1982 Jun;15(6):1144–1147. doi: 10.1128/jcm.15.6.1144-1147.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Motyl M. R., McKinley G., Janda J. M. In vitro susceptibilities of Aeromonas hydrophila, Aeromonas sobria, and Aeromonas caviae to 22 antimicrobial agents. Antimicrob Agents Chemother. 1985 Jul;28(1):151–153. doi: 10.1128/aac.28.1.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nishikawa Y., Kishi T. A modification of bile salts brilliant green agar for isolation of motile Aeromonas from foods and environmental specimens. Epidemiol Infect. 1987 Jun;98(3):331–336. doi: 10.1017/s0950268800062087. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Palumbo S. A., Maxino F., Williams A. C., Buchanan R. L., Thayer D. W. Starch-Ampicillin Agar for the Quantitative Detection of Aeromonas hydrophila. Appl Environ Microbiol. 1985 Oct;50(4):1027–1030. doi: 10.1128/aem.50.4.1027-1030.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rahim Z., Sanyal S. C., Aziz K. M., Huq M. I., Chowdhury A. A. Isolation of enterotoxigenic, hemolytic, and antibiotic-resistant Aeromonas hydrophila strains from infected fish in Bangladesh. Appl Environ Microbiol. 1984 Oct;48(4):865–867. doi: 10.1128/aem.48.4.865-867.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shimamura T., Watanabe S., Sasaki S. Enhancement of enterotoxin production by carbon dioxide in Vibrio cholerae. Infect Immun. 1985 Aug;49(2):455–456. doi: 10.1128/iai.49.2.455-456.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. von Graevenitz A., Zinterhofer L. The detection of Aeromonas hydrophila in stool specimens. Health Lab Sci. 1970 Jul;7(3):124–127. [PubMed] [Google Scholar]

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