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. Author manuscript; available in PMC: 2010 Feb 18.
Published in final edited form as: Vaccine. 2009 Jan 13;27(8):1154–1165. doi: 10.1016/j.vaccine.2008.12.035

Figure 7. Optimal antigenic reactivity of sera requires post-translationally-modified or properly-folded target proteins.

Figure 7

A. Untreated or endoglycosidase F1-treated proteins were immobilized on uncoated 384-well micro-ELISA plates and reactivity of purified IgG was assessed. B. Untreated or TFA-treated proteins were immobilized on ELISA plates for testing of reactivity of IgG from selected vaccinated individuals. In both panels, the dotted line indicates the cut-off for positivity where 0.05–0.1 is considered borderline and above 0.1 significantly positive.