Abstract
Five different enzyme immunoassays, electron microscopy, and latex agglutination (Slidex; bioMerieux) were compared for the rapid detection of human rotavirus in fecal specimens. The enzyme immunoassay using rotavirus polyclonal antiserum (Dakopatts) with simple in-house modifications was shown by the use of confirmatory tests to be the most sensitive and specific procedure.
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- Beards G. M., Campbell A. D., Cottrell N. R., Peiris J. S., Rees N., Sanders R. C., Shirley J. A., Wood H. C., Flewett T. H. Enzyme-linked immunosorbent assays based on polyclonal and monoclonal antibodies for rotavirus detection. J Clin Microbiol. 1984 Feb;19(2):248–254. doi: 10.1128/jcm.19.2.248-254.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brandt C. D., Kim H. W., Rodriguez W. J., Thomas L., Yolken R. H., Arrobio J. O., Kapikian A. Z., Parrott R. H., Chanock R. M. Comparison of direct electron microscopy, immune electron microscopy, and rotavirus enzyme-linked immunosorbent assay for detection of gastroenteritis viruses in children. J Clin Microbiol. 1981 May;13(5):976–981. doi: 10.1128/jcm.13.5.976-981.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gerna G., Sarasini A., Passarani N., Torsellini M., Parea M., Battaglia M. Comparative evaluation of a commercial enzyme-linked immunoassay and solid-phase immune electron microscopy for rotavirus detection in stool specimens. J Clin Microbiol. 1987 Jun;25(6):1137–1139. doi: 10.1128/jcm.25.6.1137-1139.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Grauballe P. C., Vestergaard B. F., Meyling A., Genner J. Optimized enzyme-linked immunosorbent assay for detection of human and bovine rotavirus in stools: Comparison with electron-microscopy, immunoelectro-osmophoresis, and fluorescent antibody techniques. J Med Virol. 1981;7(1):29–40. doi: 10.1002/jmv.1890070104. [DOI] [PubMed] [Google Scholar]
- Krause P. J., Hyams J. S., Middleton P. J., Herson V. C., Flores J. Unreliability of Rotazyme ELISA test in neonates. J Pediatr. 1983 Aug;103(2):259–262. doi: 10.1016/s0022-3476(83)80361-8. [DOI] [PubMed] [Google Scholar]
- Miotti P. G., Eiden J., Yolken R. H. Comparative efficiency of commercial immunoassays for the diagnosis of rotavirus gastroenteritis during the course of infection. J Clin Microbiol. 1985 Nov;22(5):693–698. doi: 10.1128/jcm.22.5.693-698.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Svensson L., Grandien M., Pettersson C. A. Comparison of solid-phase immune electron microscopy by use of protein A with direct electron microscopy and enzyme-linked immunosorbent assay for detection of rotavirus in stool. J Clin Microbiol. 1983 Nov;18(5):1244–1249. doi: 10.1128/jcm.18.5.1244-1249.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thomas E. E., Puterman M. L., Kawano E., Curran M. Evaluation of seven immunoassays for detection of rotavirus in pediatric stool samples. J Clin Microbiol. 1988 Jun;26(6):1189–1193. doi: 10.1128/jcm.26.6.1189-1193.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- di Natale I., Tessarin M., Sartori C. A., Patelli G., Dal Pos R., Sorato R., Dal Pozzo A., Jelmoni A. M., Corsini A. Perforazioni coliche non traumatiche in peritoneo libero. A proposito di 61 casi. Minerva Chir. 1989 Sep 30;44(18):1981–1984. [PubMed] [Google Scholar]