Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1989 Mar;27(3):448–452. doi: 10.1128/jcm.27.3.448-452.1989

Evaluation of a monoclonal antibody pool for rapid diagnosis of respiratory viral infections.

C Stout 1, M D Murphy 1, S Lawrence 1, S Julian 1
PMCID: PMC267338  PMID: 2541165

Abstract

A pool of monoclonal antibodies (MAbP) was evaluated both as a method of cell culture confirmation and as a rapid diagnostic screen for viral infection in respiratory secretions. The MAbP was used in a two-step fluorescent staining procedure on cells harvested from cultures (phase 1) and on exfoliated nasopharyngeal or tracheal cells (phase 2). Antibodies in the MAbP were directed against respiratory syncytial virus, adenoviruses, parainfluenza virus types 1, 2, and 3, and influenza viruses A and B. Individual antiviral antibody stains were used to identify specific viruses from MAbP-positive specimens. In phase 1 (cell culture confirmation only), 241 respiratory specimens were tested. MAbP sensitivity and specificity were 91 and 94%, respectively. In phase 2, 376 respiratory specimens were evaluated by direct staining of exfoliated cells, and these results were compared with results of cell culture isolation. The sensitivity and specificity of the MAbP used in direct specimen testing were 69 and 97%, respectively. These results indicate that the MAbP is highly specific and fairly sensitive for detection of seven different respiratory viruses in one testing procedure. The MAbP is a rapid screening technique for respiratory secretions and is potentially a cost-effective approach to viral detection.

Full text

PDF
448

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bell D. M., Walsh E. E., Hruska J. F., Schnabel K. C., Hall C. B. Rapid detection of respiratory syncytial virus with a monoclonal antibody. J Clin Microbiol. 1983 Jun;17(6):1099–1101. doi: 10.1128/jcm.17.6.1099-1101.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bromberg K., Tannis G., Daidone B., Clarke L., Sierra M. F. Comparison of ortho respiratory syncytial virus enzyme-linked immunosorbent assay and HEp-2 cell culture. J Clin Microbiol. 1985 Dec;22(6):1071–1072. doi: 10.1128/jcm.22.6.1071-1072.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dolin R., Reichman R. C., Madore H. P., Maynard R., Linton P. N., Webber-Jones J. A controlled trial of amantadine and rimantadine in the prophylaxis of influenza A infection. N Engl J Med. 1982 Sep 2;307(10):580–584. doi: 10.1056/NEJM198209023071002. [DOI] [PubMed] [Google Scholar]
  4. Gardner P. S., McQuillin J., McGuckin R. The late detection of respiratory syncytial virus in cells of respiratory tract by immunofluorescence. J Hyg (Lond) 1970 Dec;68(4):575–580. doi: 10.1017/s0022172400042509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Grandien M., Pettersson C. A., Gardner P. S., Linde A., Stanton A. Rapid viral diagnosis of acute respiratory infections: comparison of enzyme-linked immunosorbent assay and the immunofluorescence technique for detection of viral antigens in nasopharyngeal secretions. J Clin Microbiol. 1985 Nov;22(5):757–760. doi: 10.1128/jcm.22.5.757-760.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hall C. B., Douglas R. G., Jr, Geiman J. M., Messner M. K. Nosocomial respiratory syncytial virus infections. N Engl J Med. 1975 Dec 25;293(26):1343–1346. doi: 10.1056/NEJM197512252932604. [DOI] [PubMed] [Google Scholar]
  7. Hall C. B., McBride J. T., Walsh E. E., Bell D. M., Gala C. L., Hildreth S., Ten Eyck L. G., Hall W. J. Aerosolized ribavirin treatment of infants with respiratory syncytial viral infection. A randomized double-blind study. N Engl J Med. 1983 Jun 16;308(24):1443–1447. doi: 10.1056/NEJM198306163082403. [DOI] [PubMed] [Google Scholar]
  8. Hughes J. H., Mann D. R., Hamparian V. V. Detection of respiratory syncytial virus in clinical specimens by viral culture, direct and indirect immunofluorescence, and enzyme immunoassay. J Clin Microbiol. 1988 Mar;26(3):588–591. doi: 10.1128/jcm.26.3.588-591.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Krasinski K. Severe respiratory syncytial virus infection: clinical features, nosocomial acquisition and outcome. Pediatr Infect Dis. 1985 May-Jun;4(3):250–257. doi: 10.1097/00006454-198505000-00009. [DOI] [PubMed] [Google Scholar]
  10. Kumar M. L., Super D. M., Lembo R. M., Thomas F. C., Prokay S. L. Diagnostic efficacy of two rapid tests for detection of respiratory syncytial virus antigen. J Clin Microbiol. 1987 May;25(5):873–875. doi: 10.1128/jcm.25.5.873-875.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lauer B. A. Comparison of virus culturing and immunofluorescence for rapid detection of respiratory syncytial virus in nasopharyngeal secretions: sensitivity and specificity. J Clin Microbiol. 1982 Aug;16(2):411–412. doi: 10.1128/jcm.16.2.411-412.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lauer B. A., Masters H. A., Wren C. G., Levin M. J. Rapid detection of respiratory syncytial virus in nasopharyngeal secretions by enzyme-linked immunosorbent assay. J Clin Microbiol. 1985 Nov;22(5):782–785. doi: 10.1128/jcm.22.5.782-785.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Masters H. B., Bate B. J., Wren C., Lauer B. A. Detection of respiratory syncytial virus antigen in nasopharyngeal secretions by Abbott Diagnostics enzyme immunoassay. J Clin Microbiol. 1988 Jun;26(6):1103–1105. doi: 10.1128/jcm.26.6.1103-1105.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McClung H. W., Knight V., Gilbert B. E., Wilson S. Z., Quarles J. M., Divine G. W. Ribavirin aerosol treatment of influenza B virus infection. JAMA. 1983 May 20;249(19):2671–2674. [PubMed] [Google Scholar]
  15. Minnich L., Ray C. G. Comparison of direct immunofluorescent staining of clinical specimens for respiratory virus antigens with conventional isolation techniques. J Clin Microbiol. 1980 Sep;12(3):391–394. doi: 10.1128/jcm.12.3.391-394.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mintz L., Ballard R. A., Sniderman S. H., Roth R. S., Drew W. L. Nosocomial respiratory syncytial virus infections in an intensive care nursery: rapid diagnosis by direct immunofluorescence. Pediatrics. 1979 Aug;64(2):149–153. [PubMed] [Google Scholar]
  17. Ray C. G., Minnich L. L. Efficiency of immunofluorescence for rapid detection of common respiratory viruses. J Clin Microbiol. 1987 Feb;25(2):355–357. doi: 10.1128/jcm.25.2.355-357.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shalit I., McKee P. A., Beauchamp H., Waner J. L. Comparison of polyclonal antiserum versus monoclonal antibodies for the rapid diagnosis of influenza A virus infections by immunofluorescence in clinical specimens. J Clin Microbiol. 1985 Nov;22(5):877–879. doi: 10.1128/jcm.22.5.877-879.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stokes C. E., Bernstein J. M., Kyger S. A., Hayden F. G. Rapid diagnosis of influenza A and B by 24-h fluorescent focus assays. J Clin Microbiol. 1988 Jul;26(7):1263–1266. doi: 10.1128/jcm.26.7.1263-1266.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Swenson P. D., Kaplan M. H. Rapid detection of respiratory syncytial virus in nasopharyngeal aspirates by a commercial enzyme immunoassay. J Clin Microbiol. 1986 Mar;23(3):485–488. doi: 10.1128/jcm.23.3.485-488.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Waner J. L., Whitehurst N. J., Jonas S., Wall L., Shalaby H. Isolation of viruses from specimens submitted for direct immunofluorescence test for respiratory syncytial virus. J Pediatr. 1986 Feb;108(2):249–250. doi: 10.1016/s0022-3476(86)80993-3. [DOI] [PubMed] [Google Scholar]
  22. Wong D. T., Welliver R. C., Riddlesberger K. R., Sun M. S., Ogra P. L. Rapid diagnosis of parainfluenza virus infection in children. J Clin Microbiol. 1982 Jul;16(1):164–167. doi: 10.1128/jcm.16.1.164-167.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES