Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1989 Mar;27(3):486–489. doi: 10.1128/jcm.27.3.486-489.1989

Comparison of core antigen (p24) assay and reverse transcriptase activity for detection of human immunodeficiency virus type 1 replication.

S Land 1, F Beaton 1, D A McPhee 1, I D Gust 1
PMCID: PMC267344  PMID: 2469698

Abstract

This report compares two assay systems for monitoring human immunodeficiency virus (HIV) replication in peripheral blood leukocyte cultures. A commercial enzyme-linked immunoassay detected core antigen (p24) in 80% of cell cultures from HIV-seropositive individuals, whereas 67% of the cell cultures produced detectable levels of reverse transcriptase activity. There were clearly three patterns of reverse transcriptase activity produced, two of which may evade detection without regular sampling and maintaining cell cultures for more than 4 weeks. Once established, core antigen levels remained high so that cell cultures could be confidently monitored by an intermittent screening regimen.

Full text

PDF
486

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albert J., Gaines H., Sönnerborg A., Nyström G., Pehrson P. O., Chiodi F., von Sydow M., Moberg L., Lidman K., Christensson B. Isolation of human immunodeficiency virus (HIV) from plasma during primary HIV infection. J Med Virol. 1987 Sep;23(1):67–73. doi: 10.1002/jmv.1890230108. [DOI] [PubMed] [Google Scholar]
  2. Bäcker U., Weinauer F., Gathof G., Eberle J. HIV antigen screening in blood donors. Lancet. 1987 Nov 21;2(8569):1213–1214. doi: 10.1016/s0140-6736(87)91353-5. [DOI] [PubMed] [Google Scholar]
  3. Coffin J. M. Genetic variation in AIDS viruses. Cell. 1986 Jul 4;46(1):1–4. doi: 10.1016/0092-8674(86)90851-2. [DOI] [PubMed] [Google Scholar]
  4. Feorino P., Forrester B., Schable C., Warfield D., Schochetman G. Comparison of antigen assay and reverse transcriptase assay for detecting human immunodeficiency virus in culture. J Clin Microbiol. 1987 Dec;25(12):2344–2346. doi: 10.1128/jcm.25.12.2344-2346.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gaines H., von Sydow M., Sönnerborg A., Albert J., Czajkowski J., Pehrson P. O., Chiodi F., Moberg L., Fenyö E. M., Asjö B. Antibody response in primary human immunodeficiency virus infection. Lancet. 1987 May 30;1(8544):1249–1253. doi: 10.1016/s0140-6736(87)92696-1. [DOI] [PubMed] [Google Scholar]
  6. Gallo R. C., Salahuddin S. Z., Popovic M., Shearer G. M., Kaplan M., Haynes B. F., Palker T. J., Redfield R., Oleske J., Safai B. Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS. Science. 1984 May 4;224(4648):500–503. doi: 10.1126/science.6200936. [DOI] [PubMed] [Google Scholar]
  7. Gupta P., Balachandran R., Grovit K., Webster D., Rinaldo C., Jr Detection of human immunodeficiency virus by reverse transcriptase assay, antigen capture assay, and radioimmunoassay. J Clin Microbiol. 1987 Jun;25(6):1122–1125. doi: 10.1128/jcm.25.6.1122-1125.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Healey D. S., Jowett J. B., Beaton F., Maskill W. J., Gust I. D. Comparison of enzyme immunoassay and reverse transcriptase assay for detection of HIV in culture supernates. J Virol Methods. 1987 Sep;17(3-4):237–245. doi: 10.1016/0166-0934(87)90134-0. [DOI] [PubMed] [Google Scholar]
  9. Healey D. S., Maskill W. J., Neate E. V., Beaton F., Gust I. D. A preliminary evaluation of five HIV antigen detection assays. J Virol Methods. 1988 Jun;20(2):115–125. doi: 10.1016/0166-0934(88)90145-0. [DOI] [PubMed] [Google Scholar]
  10. Kitchen L. W., Barin F., Sullivan J. L., McLane M. F., Brettler D. B., Levine P. H., Essex M. Aetiology of AIDS--antibodies to human T-cell leukaemia virus (type III) in haemophiliacs. Nature. 1984 Nov 22;312(5992):367–369. doi: 10.1038/312367a0. [DOI] [PubMed] [Google Scholar]
  11. Miyoshi I., Kubonishi I., Yoshimoto S., Akagi T., Ohtsuki Y., Shiraishi Y., Nagata K., Hinuma Y. Type C virus particles in a cord T-cell line derived by co-cultivating normal human cord leukocytes and human leukaemic T cells. Nature. 1981 Dec 24;294(5843):770–771. doi: 10.1038/294770a0. [DOI] [PubMed] [Google Scholar]
  12. Neate E. V., Pringle R. C., Jowett J. B., Healey D. S., Gust I. D. Isolation of HIV from Australian patients with AIDS, AIDS related conditions and healthy antibody positive individuals. Aust N Z J Med. 1987 Oct;17(5):461–466. doi: 10.1111/j.1445-5994.1987.tb00096.x. [DOI] [PubMed] [Google Scholar]
  13. Paul D. A., Falk L. A., Kessler H. A., Chase R. M., Blaauw B., Chudwin D. S., Landay A. L. Correlation of serum HIV antigen and antibody with clinical status in HIV-infected patients. J Med Virol. 1987 Aug;22(4):357–363. doi: 10.1002/jmv.1890220408. [DOI] [PubMed] [Google Scholar]
  14. Ranki A., Valle S. L., Krohn M., Antonen J., Allain J. P., Leuther M., Franchini G., Krohn K. Long latency precedes overt seroconversion in sexually transmitted human-immunodeficiency-virus infection. Lancet. 1987 Sep 12;2(8559):589–593. doi: 10.1016/s0140-6736(87)92985-0. [DOI] [PubMed] [Google Scholar]
  15. Ulrich P. P., Busch M. P., el-Beik T., Shiota J., Vennari J., Shriver K., Vyas G. N. Assessment of human immunodeficiency virus expression in cocultures of peripheral blood mononuclear cells from healthy seropositive subjects. J Med Virol. 1988 May;25(1):1–10. doi: 10.1002/jmv.1890250102. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES