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. 1989 Mar;27(3):580–582. doi: 10.1128/jcm.27.3.580-582.1989

Detection of antibodies to caprine arthritis-encephalitis virus by protein G enzyme-linked immunosorbent assay and immunoblotting.

R Zanoni 1, A Krieg 1, E Peterhans 1
PMCID: PMC267367  PMID: 2541170

Abstract

Sera from goats suffering from caprine arthritis-encephalitis contained antibodies to virus proteins of 15, 17, 28, 40, and 130 kilodaltons in immunoblots of maedi-visna virus. We propose to use immunoblotting as a validation test for enzyme-linked immunosorbent assay and demonstrate that the specificity of indirect enzyme-linked immunosorbent assay can be improved by replacing second antibody by a protein G-avidin-biotin conjugate.

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Selected References

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