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. 1989 Mar;27(3):586–588. doi: 10.1128/jcm.27.3.586-588.1989

Experience with an enzyme immunoassay for serotyping human group A rotaviruses.

L E Unicomb 1, B S Coulson 1, R F Bishop 1
PMCID: PMC267369  PMID: 2541171

Abstract

An enzyme immunoassay utilizing neutralizing monoclonal antibodies to VP7 of four human group A rotavirus serotypes successfully typed rotaviruses in 71.4% (568 of 796) of fecal specimens. Sensitivity was enhanced by using homologous capture and detector antibodies. Serotyping was most successful with specimens stored for less than 3 years and containing 10(4) or more particles per ml.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albert M. J., Bishop R. F. Cultivation of human rotaviruses in cell culture. J Med Virol. 1984;13(4):377–383. doi: 10.1002/jmv.1890130409. [DOI] [PubMed] [Google Scholar]
  2. Bastardo J. W., McKimm-Breschkin J. L., Sonza S., Mercer L. D., Holmes I. H. Preparation and characterization of antisera to electrophoretically purified SA11 virus polypeptides. Infect Immun. 1981 Dec;34(3):641–647. doi: 10.1128/iai.34.3.641-647.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Coulson B. S., Fowler K. J., Bishop R. F., Cotton R. G. Neutralizing monoclonal antibodies to human rotavirus and indications of antigenic drift among strains from neonates. J Virol. 1985 Apr;54(1):14–20. doi: 10.1128/jvi.54.1.14-20.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Coulson B. S., Fowler K. J., White J. R., Cotton R. G. Non-neutralizing monoclonal antibodies to a trypsin-sensitive site on the major glycoprotein of rotavirus which discriminate between virus serotypes. Arch Virol. 1987;93(3-4):199–211. doi: 10.1007/BF01310974. [DOI] [PubMed] [Google Scholar]
  5. Coulson B. S., Holmes I. H. An improved enzyme-linked immunosorbent assay for the detection of rotavirus in faeces of neonates. J Virol Methods. 1984 May;8(3):165–179. doi: 10.1016/0166-0934(84)90011-9. [DOI] [PubMed] [Google Scholar]
  6. Coulson B. S., Tursi J. M., McAdam W. J., Bishop R. F. Derivation of neutralizing monoclonal antibodies to human rotaviruses and evidence that an immunodominant neutralization site is shared between serotypes 1 and 3. Virology. 1986 Oct 30;154(2):302–312. doi: 10.1016/0042-6822(86)90456-3. [DOI] [PubMed] [Google Scholar]
  7. Gerna G., Sarasini A., Coulson B. S., Parea M., Torsellini M., Arbustini E., Battaglia M. Comparative sensitivities of solid-phase immune electron microscopy and enzyme-linked immunosorbent assay for serotyping of human rotavirus strains with neutralizing monoclonal antibodies. J Clin Microbiol. 1988 Jul;26(7):1383–1387. doi: 10.1128/jcm.26.7.1383-1387.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Green K. Y., Sears J. F., Taniguchi K., Midthun K., Hoshino Y., Gorziglia M., Nishikawa K., Urasawa S., Kapikian A. Z., Chanock R. M. Prediction of human rotavirus serotype by nucleotide sequence analysis of the VP7 protein gene. J Virol. 1988 May;62(5):1819–1823. doi: 10.1128/jvi.62.5.1819-1823.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Whitby H. J., Rodgers F. G. Detection of virus particles by electron microscopy with polyacrylamide hydrogel. J Clin Pathol. 1980 May;33(5):484–487. doi: 10.1136/jcp.33.5.484. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. de Zoysa I., Feachem R. G. Interventions for the control of diarrhoeal diseases among young children: rotavirus and cholera immunization. Bull World Health Organ. 1985;63(3):569–583. [PMC free article] [PubMed] [Google Scholar]

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