Abstract
A total of 201 endocervical specimens were obtained from patients with a clinical or epidemiological history suggestive of chlamydial infection. These specimens were tested by DNA probe (Gen-Probe, San Diego, Calif.) and the IDEIA III (Boots-Celltech, Berkshire, United Kingdom) monoclonal antibody enzyme immunoassay and compared with cell culture for detection of Chlamydia trachomatis. Discrepancies between cell culture and antigen detection methods were resolved by direct fluorescent-antibody testing. In a population with a 17.4% prevalence, the sensitivities and specificities of these assays were 82.8 and 99.4%, respectively, for the DNA probe assay and 97.1 and 98.1%, respectively, for the IDEIA III.
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Selected References
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- Grillner L., Beckman S., Hammar H. Comparison of two enzyme immunoassays and an immunofluorescence test for detection of Chlamydia trachomatis. Eur J Clin Microbiol. 1986 Oct;5(5):559–562. doi: 10.1007/BF02017705. [DOI] [PubMed] [Google Scholar]
- Kobayashi T. K., Ueda M., Araki H., Toyoda K., Ohmori K., Sawaragi I. Immunocytochemical demonstration of Chlamydia infection in the urogenital tracts. Diagn Cytopathol. 1987 Dec;3(4):302–306. doi: 10.1002/dc.2840030408. [DOI] [PubMed] [Google Scholar]
- Lefebvre J., Laperrière H., Rousseau H., Massé R. Comparison of three techniques for detection of Chlamydia trachomatis in endocervical specimens from asymptomatic women. J Clin Microbiol. 1988 Apr;26(4):726–731. doi: 10.1128/jcm.26.4.726-731.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Phillips R. S., Hanff P. A., Kauffman R. S., Aronson M. D. Use of a direct fluorescent antibody test for detecting Chlamydia trachomatis cervical infection in women seeking routine gynecologic care. J Infect Dis. 1987 Oct;156(4):575–581. doi: 10.1093/infdis/156.4.575. [DOI] [PubMed] [Google Scholar]
- Pugh S. F., Slack R. C., Caul E. O., Paul I. D., Appleton P. N., Gatley S. Enzyme amplified immunoassay: a novel technique applied to direct detection of Chlamydia trachomatis in clinical specimens. J Clin Pathol. 1985 Oct;38(10):1139–1141. doi: 10.1136/jcp.38.10.1139. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ryan R. W., Kwasnik I., Steingrimsson O., Gudmundsson J., Thorarinsson H., Tilton R. C. Rapid detection of Chlamydia trachomatis by an enzyme immunoassay method. Diagn Microbiol Infect Dis. 1986 Sep;5(3):225–234. doi: 10.1016/0732-8893(86)90006-4. [DOI] [PubMed] [Google Scholar]
- Schachter J. Immunodiagnosis of sexually transmitted disease. Yale J Biol Med. 1985 Sep-Oct;58(5):443–452. [PMC free article] [PubMed] [Google Scholar]
- Schachter J. Rapid diagnosis of sexually transmitted diseases--speed has a price. Diagn Microbiol Infect Dis. 1986 Mar;4(3):185–189. doi: 10.1016/0732-8893(86)90097-0. [DOI] [PubMed] [Google Scholar]
- Smith J. W., Rogers R. E., Katz B. P., Brickler J. F., Lineback P. L., Van der Pol B., Jones R. B. Diagnosis of chlamydial infection in women attending antenatal and gynecologic clinics. J Clin Microbiol. 1987 May;25(5):868–872. doi: 10.1128/jcm.25.5.868-872.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tilton R. C., Judson F. N., Barnes R. C., Gruninger R. P., Ryan R. W., Steingrimsson O. Multicenter comparative evaluation of two rapid microscopic methods and culture for detection of Chlamydia trachomatis in patient specimens. J Clin Microbiol. 1988 Feb;26(2):167–170. doi: 10.1128/jcm.26.2.167-170.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tjiam K. H., van Heijst B. Y., van Zuuren A., Wagenvoort J. H., van Joost T., Stolz E., Michel M. F. Evaluation of an enzyme immunoassay for the diagnosis of chlamydial infections in urogenital specimens. J Clin Microbiol. 1986 Apr;23(4):752–754. doi: 10.1128/jcm.23.4.752-754.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]