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VCI assay for S. cerevisiae Snf1:Snf4 and Pbs2:Hog1. An S. cerevisiae vps4Δ strain was transformed with a Snf1-GFP fusion plasmid and either a Vps32 vector (A) or a Snf4-Vps32 fusion plasmid (B). The vps4Δ strain was independently transformed with a Pbs2-GFP fusion plasmid and either a Vps32 vector (C) or a Hog1-Vps32 fusion plasmid (D). GFP fluorescence images are shown.
