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. 1989 Jun;27(6):1151–1154. doi: 10.1128/jcm.27.6.1151-1154.1989

Evaluation of the Abbott TESTPACK RSV enzyme immunoassay for detection of respiratory syncytial virus in nasopharyngeal swab specimens.

E M Swierkosz 1, R Flanders 1, L Melvin 1, J D Miller 1, M W Kline 1
PMCID: PMC267517  PMID: 2666434

Abstract

The Abbott TESTPACK RSV assay (Abbott Laboratories, North Chicago, Ill.), a rapid (20-min) enzyme immunoassay, was compared with culture and direct immunofluorescence (DFA) of nasopharyngeal cells for the detection of respiratory syncytial virus (RSV) in nasopharyngeal swab specimens. Nasopharyngeal swab specimens, collected from 234 infants, were placed in viral transport medium. Portions of specimen in transport medium were used for each test. Of 234 specimens, 70 (30%) were culture positive, 103 (44%) were DFA positive, 107 (46%) were culture or DFA positive, and 112 (48%) were TESTPACK RSV positive. Of 19 specimens positive by TESTPACK RSV but negative by culture or DFA, 15 were positive by the blocking assay. A total of 122 specimens were culture, DFA, or blocking assay positive; TESTPACK RSV detected 108 specimens (sensitivity, 89%). The specificity, positive predictive value, and negative predictive value of TESTPACK RSV as compared with those of culture, DFA, and the blocking assay were 96, 96, and 89%, respectively. By comparison, the sensitivity, specificity, positive predictive value, and negative predictive value of combined culture and DFA were 88, 100, 100, and 88%, respectively. TESTPACK RSV is a rapid and reliable enzyme immunoassay for the direct detection of RSV antigen in nasopharyngeal swab specimens.

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Selected References

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  1. Ahluwalia G. S., Hammond G. W. Comparison of cell culture and three enzyme-linked immunosorbent assays for the rapid diagnosis of respiratory syncytial virus from nasopharyngeal aspirate and tracheal secretion specimens. Diagn Microbiol Infect Dis. 1988 Mar;9(3):187–192. doi: 10.1016/0732-8893(88)90028-4. [DOI] [PubMed] [Google Scholar]
  2. Ahluwalia G., Embree J., McNicol P., Law B., Hammond G. W. Comparison of nasopharyngeal aspirate and nasopharyngeal swab specimens for respiratory syncytial virus diagnosis by cell culture, indirect immunofluorescence assay, and enzyme-linked immunosorbent assay. J Clin Microbiol. 1987 May;25(5):763–767. doi: 10.1128/jcm.25.5.763-767.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Arens M. Q., Swierkosz E. M., Schmidt R. R., Armstrong T., Rivetna K. A. Enhanced isolation of respiratory syncytial virus in cell culture. J Clin Microbiol. 1986 Apr;23(4):800–802. doi: 10.1128/jcm.23.4.800-802.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Arens M. Q., Swierkosz E. M., Schmidt R. R., Armstrong T., Rivetna K. A. Strategy for efficient detection of respiratory viruses in pediatric clinical specimens. Diagn Microbiol Infect Dis. 1986 Nov;5(4):307–312. doi: 10.1016/0732-8893(86)90033-7. [DOI] [PubMed] [Google Scholar]
  5. Brandt C. D., Kim H. W., Arrobio J. O., Jeffries B. C., Wood S. C., Chanock R. M., Parrott R. H. Epidemiology of respiratory syncytial virus infection in Washington, D.C. 3. Composite analysis of eleven consecutive yearly epidemics. Am J Epidemiol. 1973 Nov;98(5):355–364. doi: 10.1093/oxfordjournals.aje.a121565. [DOI] [PubMed] [Google Scholar]
  6. Bromberg K., Tannis G., Daidone B., Clarke L., Sierra M. F. Comparison of ortho respiratory syncytial virus enzyme-linked immunosorbent assay and HEp-2 cell culture. J Clin Microbiol. 1985 Dec;22(6):1071–1072. doi: 10.1128/jcm.22.6.1071-1072.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bromberg K., Tannis G., Daidone B., Clarke L., Sierra M. Comparison of HEp-2 cell culture and Abbott respiratory syncytial virus enzyme immunoassay. J Clin Microbiol. 1987 Feb;25(2):434–436. doi: 10.1128/jcm.25.2.434-436.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Freymuth F., Quibriac M., Petitjean J., Amiel M. L., Pothier P., Denis A., Duhamel J. F. Comparison of two new tests for rapid diagnosis of respiratory syncytial virus infections by enzyme-linked immunosorbent assay and immunofluorescence techniques. J Clin Microbiol. 1986 Dec;24(6):1013–1016. doi: 10.1128/jcm.24.6.1013-1016.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gardner P. S., McQuillin J., McGuckin R. The late detection of respiratory syncytial virus in cells of respiratory tract by immunofluorescence. J Hyg (Lond) 1970 Dec;68(4):575–580. doi: 10.1017/s0022172400042509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kumar M. L., Super D. M., Lembo R. M., Thomas F. C., Prokay S. L. Diagnostic efficacy of two rapid tests for detection of respiratory syncytial virus antigen. J Clin Microbiol. 1987 May;25(5):873–875. doi: 10.1128/jcm.25.5.873-875.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lauer B. A., Masters H. A., Wren C. G., Levin M. J. Rapid detection of respiratory syncytial virus in nasopharyngeal secretions by enzyme-linked immunosorbent assay. J Clin Microbiol. 1985 Nov;22(5):782–785. doi: 10.1128/jcm.22.5.782-785.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Masters H. B., Bate B. J., Wren C., Lauer B. A. Detection of respiratory syncytial virus antigen in nasopharyngeal secretions by Abbott Diagnostics enzyme immunoassay. J Clin Microbiol. 1988 Jun;26(6):1103–1105. doi: 10.1128/jcm.26.6.1103-1105.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Masters H. B., Weber K. O., Groothuis J. R., Wren C. G., Lauer B. A. Comparison of nasopharyngeal washings and swab specimens for diagnosis of respiratory syncytial virus by EIA, FAT, and cell culture. Diagn Microbiol Infect Dis. 1987 Oct;8(2):101–105. doi: 10.1016/0732-8893(87)90156-8. [DOI] [PubMed] [Google Scholar]
  14. McIntosh K., Hendry R. M., Fahnestock M. L., Pierik L. T. Enzyme-linked immunosorbent assay for detection of respiratory syncytial virus infection: application to clinical samples. J Clin Microbiol. 1982 Aug;16(2):329–333. doi: 10.1128/jcm.16.2.329-333.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Minnich L., Ray C. G. Comparison of direct immunofluorescent staining of clinical specimens for respiratory virus antigens with conventional isolation techniques. J Clin Microbiol. 1980 Sep;12(3):391–394. doi: 10.1128/jcm.12.3.391-394.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Swenson P. D., Kaplan M. H. Rapid detection of respiratory syncytial virus in nasopharyngeal aspirates by a commercial enzyme immunoassay. J Clin Microbiol. 1986 Mar;23(3):485–488. doi: 10.1128/jcm.23.3.485-488.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]

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