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. 1989 Jun;27(6):1272–1276. doi: 10.1128/jcm.27.6.1272-1276.1989

Enterotoxin production and serogroups of Campylobacter jejuni and Campylobacter coli from patients with diarrhea and from healthy laying hens.

G B Lindblom 1, B Kaijser 1, E Sjögren 1
PMCID: PMC267540  PMID: 2754001

Abstract

Enterotoxin production, a possible virulence factor, was determined in Campylobacter jejuni and Campylobacter coli by two different techniques, the CHO cell test and the GM1 enzyme-linked immunosorbent assay. The frequency of enterotoxigenic Campylobacter strains was 32% in strains from both humans with acute enteritis and healthy laying hens, as measured by the CHO cell test. The CHO cell test was significantly more sensitive than the GM1 enzyme-linked immunosorbent assay in the detection of enterotoxigenic strains. Enterotoxin production was compared with the presence of heat-stable and heat-labile antigens. There was no significant correlation between enterotoxin production and serogroups for C. jejuni or C. coli. The difference in enterotoxigenicity between C. jejuni (34.1%) and C. coli (21.9%) was not significant.

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Selected References

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  1. Black R. E., Merson M. H., Huq I., Alim A. R., Yunus M. Incidence and severity of rotavirus and Escherichia coli diarrhoea in rural Bangladesh. Implications for vaccine development. Lancet. 1981 Jan 17;1(8212):141–143. doi: 10.1016/s0140-6736(81)90719-4. [DOI] [PubMed] [Google Scholar]
  2. Blaser M. J., Berkowitz I. D., LaForce F. M., Cravens J., Reller L. B., Wang W. L. Campylobacter enteritis: clinical and epidemiologic features. Ann Intern Med. 1979 Aug;91(2):179–185. doi: 10.7326/0003-4819-91-2-179. [DOI] [PubMed] [Google Scholar]
  3. Blaser M. J., Glass R. I., Huq M. I., Stoll B., Kibriya G. M., Alim A. R. Isolation of Campylobacter fetus subsp. jejuni from Bangladeshi children. J Clin Microbiol. 1980 Dec;12(6):744–747. doi: 10.1128/jcm.12.6.744-747.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Blaser M. J., LaForce F. M., Wilson N. A., Wang W. L. Reservoirs for human campylobacteriosis. J Infect Dis. 1980 May;141(5):665–669. doi: 10.1093/infdis/141.5.665. [DOI] [PubMed] [Google Scholar]
  5. Blaser M. J., Taylor D. N., Feldman R. A. Epidemiology of Campylobacter jejuni infections. Epidemiol Rev. 1983;5:157–176. doi: 10.1093/oxfordjournals.epirev.a036256. [DOI] [PubMed] [Google Scholar]
  6. Brouwer R., Mertens M. J., Siem T. H., Katchaki J. An explosive outbreak of Campylobacter enteritis in soldiers. Antonie Van Leeuwenhoek. 1979;45(3):517–519. doi: 10.1007/BF00443293. [DOI] [PubMed] [Google Scholar]
  7. Butzler J. P., Dekeyser P., Detrain M., Dehaen F. Related vibrio in stools. J Pediatr. 1973 Mar;82(3):493–495. doi: 10.1016/s0022-3476(73)80131-3. [DOI] [PubMed] [Google Scholar]
  8. Butzler J. P., Skirrow M. B. Campylobacter enteritis. Clin Gastroenterol. 1979 Sep;8(3):737–765. [PubMed] [Google Scholar]
  9. Drake A. A., Gilchrist M. J., Washington J. A., 2nd, Huizenga K. A., Van Scoy R. E. Diarrhea due to Campylobacter fetus subspecies jejuni. A clinical review of 63 cases. Mayo Clin Proc. 1981 Jul;56(7):414–423. [PubMed] [Google Scholar]
  10. Grant I. H., Richardson N. J., Bokkenheuser V. D. Broiler chickens as potential source of Campylobacter infections in humans. J Clin Microbiol. 1980 May;11(5):508–510. doi: 10.1128/jcm.11.5.508-510.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Guerrant R. L., Brunton L. L., Schnaitman T. C., Rebhun L. I., Gilman A. G. Cyclic adenosine monophosphate and alteration of Chinese hamster ovary cell morphology: a rapid, sensitive in vitro assay for the enterotoxins of Vibrio cholerae and Escherichia coli. Infect Immun. 1974 Aug;10(2):320–327. doi: 10.1128/iai.10.2.320-327.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Guerrant R. L., Lahita R. G., Winn W. C., Jr, Roberts R. B. Campylobacteriosis in man: pathogenic mechanisms and review of 91 bloodstream infections. Am J Med. 1978 Oct;65(4):584–592. doi: 10.1016/0002-9343(78)90845-8. [DOI] [PubMed] [Google Scholar]
  13. Hwang M. N., Ederer G. M. Rapid hippurate hydrolysis method for presumptive identification of group B streptococci. J Clin Microbiol. 1975 Jan;1(1):114–115. doi: 10.1128/jcm.1.1.114-115.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Johnson W. M., Lior H. Cytotoxic and cytotonic factors produced by Campylobacter jejuni, Campylobacter coli, and Campylobacter laridis. J Clin Microbiol. 1986 Aug;24(2):275–281. doi: 10.1128/jcm.24.2.275-281.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kaijser B., Svanborg-Edén C., Wadström T. High frequency of K15 antigen in enterotoxigenic Escherichia coli: lack of correlation to adherence to epithelial cells or sensitivity to bactericidal effect of normal serum. Zentralbl Bakteriol Orig A. 1978 Nov;242(1):17–22. [PubMed] [Google Scholar]
  16. Klipstein F. A., Engert R. F. Immunological relationship of the B subunits of Campylobacter jejuni and Escherichia coli heat-labile enterotoxins. Infect Immun. 1985 Jun;48(3):629–633. doi: 10.1128/iai.48.3.629-633.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Klipstein F. A., Engert R. F. Properties of crude Campylobacter jejuni heat-labile enterotoxin. Infect Immun. 1984 Aug;45(2):314–319. doi: 10.1128/iai.45.2.314-319.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Klipstein F. A., Engert R. F., Short H., Schenk E. A. Pathogenic properties of Campylobacter jejuni: assay and correlation with clinical manifestations. Infect Immun. 1985 Oct;50(1):43–49. doi: 10.1128/iai.50.1.43-49.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lindblom G. B., Sjörgren E., Kaijser B. Natural campylobacter colonization in chickens raised under different environmental conditions. J Hyg (Lond) 1986 Jun;96(3):385–391. doi: 10.1017/s0022172400066146. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Lior H., Woodward D. L., Edgar J. A., Laroche L. J., Gill P. Serotyping of Campylobacter jejuni by slide agglutination based on heat-labile antigenic factors. J Clin Microbiol. 1982 May;15(5):761–768. doi: 10.1128/jcm.15.5.761-768.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Park C. E., Stankiewicz Z. K., Lovett J., Hunt J. Incidence of Campylobacter jejuni in fresh eviscerated whole market chickens. Can J Microbiol. 1981 Aug;27(8):841–842. doi: 10.1139/m81-129. [DOI] [PubMed] [Google Scholar]
  22. Penner J. L., Hennessy J. N., Congi R. V. Serotyping of Campylobacter jejuni and Campylobacter coli on the basis of thermostable antigens. Eur J Clin Microbiol. 1983 Aug;2(4):378–383. doi: 10.1007/BF02019474. [DOI] [PubMed] [Google Scholar]
  23. Penner J. L., Hennessy J. N. Passive hemagglutination technique for serotyping Campylobacter fetus subsp. jejuni on the basis of soluble heat-stable antigens. J Clin Microbiol. 1980 Dec;12(6):732–737. doi: 10.1128/jcm.12.6.732-737.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Ruiz-Palacios G. M., Torres J., Torres N. I., Escamilla E., Ruiz-Palacios B. R., Tamayo J. Cholera-like enterotoxin produced by Campylobacter jejuni. Characterisation and clinical significance. Lancet. 1983 Jul 30;2(8344):250–253. doi: 10.1016/s0140-6736(83)90234-9. [DOI] [PubMed] [Google Scholar]
  25. Skirrow M. B. Campylobacter enteritis: a "new" disease. Br Med J. 1977 Jul 2;2(6078):9–11. doi: 10.1136/bmj.2.6078.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Svedhem A., Kaijser B. Campylobacter fetus subspecies jejuni: a common cause of diarrhea in Sweden. J Infect Dis. 1980 Sep;142(3):353–359. doi: 10.1093/infdis/142.3.353. [DOI] [PubMed] [Google Scholar]
  27. Svedhem A., Kaijser B., MacDowall I. Intestinal occurrence of Campylobacter fetus subspecies jejuni and Clostridium difficile in children in Sweden. Eur J Clin Microbiol. 1982 Feb;1(1):29–32. doi: 10.1007/BF02014137. [DOI] [PubMed] [Google Scholar]
  28. Walder M. Epidemiology of campylobacter enteritis. Scand J Infect Dis. 1982;14(1):27–33. doi: 10.3109/inf.1982.14.issue-1.06. [DOI] [PubMed] [Google Scholar]

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