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. 1989 Jul;27(7):1538–1542. doi: 10.1128/jcm.27.7.1538-1542.1989

Application of gas-liquid chromatography to the routine identification of nonfermenting gram-negative bacteria in clinical specimens.

A Veys 1, W Callewaert 1, E Waelkens 1, K Van den Abbeele 1
PMCID: PMC267611  PMID: 2768442

Abstract

A total of 430 strains of glucose-nonfermenting gram-negative bacteria representing 35 species were analyzed for their cellular fatty acid composition by gas-liquid chromatography (GLC). On the basis of qualitative differences in their cellular fatty acid composition, these bacteria could be divided into 19 distinct chromatographic groups. Eight Pseudomonas species, Achromobacter xylosoxidans, group Vd, and Agrobacterium radiobacter were identified from their fatty acid compositions alone. The other glucose-nonfermenting gram-negative bacterial species studied here, classified within nine distinct GLC groups, were easily recognized by using the GLC fatty acid analysis supplemented with a limited number of conventional biochemical tests. The results support the hypothesis that bacterial fatty acid composition is rather specific and that qualitative GLC fatty acid analysis can be adapted in the clinical laboratory either to provide additional criteria for differentiation of closely related groups or to serve as a rapid and highly reproducible method for their routine identification.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABEL K., DESCHMERTZING H., PETERSON J. I. CLASSIFICATION OF MICROORGANISMS BY ANALYSIS OF CHEMICAL COMPOSITION. I. FEASIBILITY OF UTILIZING GAS CHROMATOGRAPHY. J Bacteriol. 1963 May;85:1039–1044. doi: 10.1128/jb.85.5.1039-1044.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dees S. B., Moss C. W. Cellular fatty acids of Alcaligenes and Pseudomonas species isolated from clinical specimens. J Clin Microbiol. 1975 May;1(5):414–419. doi: 10.1128/jcm.1.5.414-419.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dees S. B., Moss C. W. Identification of Achromobacter species by cellular fatty acids and by production of keto acids. J Clin Microbiol. 1978 Jul;8(1):61–66. doi: 10.1128/jcm.8.1.61-66.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dees S. B., Moss C. W., Weaver R. E., Hollis D. Cellular fatty acid composition of Pseudomonas paucimobilis and groups IIk-2, Ve-1, and Ve-2. J Clin Microbiol. 1979 Aug;10(2):206–209. doi: 10.1128/jcm.10.2.206-209.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Freney J., Hansen W., Etienne J., Vandenesch F., Fleurette J. Postoperative infant septicemia caused by Pseudomonas luteola (CDC group Ve-1) and Pseudomonas oryzihabitans (CDC group Ve-2). J Clin Microbiol. 1988 Jun;26(6):1241–1243. doi: 10.1128/jcm.26.6.1241-1243.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jantzen E., Bryn K., Bergan T., Bovre K. Gas chromatography of bacterial whole cell methanolysates. VII. Fatty acid composition of Acinetobacter in relation to the taxonomy of Neisseriaceae. Acta Pathol Microbiol Scand Suppl. 1975 Dec;83(6):569–580. [PubMed] [Google Scholar]
  7. Jantzen E., Bryn K., Bergan T., Bovre K. Gas chromatography of bacterial whole cell methanolysates; V. Fatty acid composition of Neisseriae and Moraxellae. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Dec;82(6):767–779. doi: 10.1111/j.1699-0463.1974.tb02374.x. [DOI] [PubMed] [Google Scholar]
  8. Kaneda T. Fatty acids in the genus Bacillus. I. Iso- and anteiso-fatty acids as characteristic constituents of lipids in 10 species. J Bacteriol. 1967 Mar;93(3):894–903. doi: 10.1128/jb.93.3.894-903.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Moss C. W., Dees S. B. Cellular fatty acids and metabolic products of Pseudomonas species obtained from clinical specimens. J Clin Microbiol. 1976 Dec;4(6):492–502. doi: 10.1128/jcm.4.6.492-502.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Moss C. W., Dees S. B. Cellular fatty acids of Flavobacterium meningosepticum and Flavobacterium species group IIb. J Clin Microbiol. 1978 Dec;8(6):772–774. doi: 10.1128/jcm.8.6.772-774.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Moss C. W., Dees S. B., Guerrant G. O. Gas-liquid chromatography of bacterial fatty acids with a fused-silica capillary column. J Clin Microbiol. 1980 Jul;12(1):127–130. doi: 10.1128/jcm.12.1.127-130.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Moss C. W., Samuels S. B., Liddle J., McKinney R. M. Occurrence of branched-cahin hydroxy fatty acids in Pseudomonas maltophilia. J Bacteriol. 1973 Jun;114(3):1018–1024. doi: 10.1128/jb.114.3.1018-1024.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Moss C. W., Samuels S. B., Weaver R. E. Cellular fatty acid composition of selected Pseudomonas species. Appl Microbiol. 1972 Oct;24(4):596–598. doi: 10.1128/am.24.4.596-598.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Moss C. W., Wallace P. L., Hollis D. G., Weaver R. E. Cultural and chemical characterization of CDC groups EO-2, M-5, and M-6, Moraxella (Moraxella) species, Oligella urethralis, Acinetobacter species, and Psychrobacter immobilis. J Clin Microbiol. 1988 Mar;26(3):484–492. doi: 10.1128/jcm.26.3.484-492.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

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