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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Circulation. 2008 Oct 20;118(19):1979–1988. doi: 10.1161/CIRCULATIONAHA.108.788331

Figure 1. IPC increases the expression of caveolae and the enrichment of protein and cholesterol in buoyant fractions.

Figure 1

Hearts were subjected to a 5 min IPC stimulus. Control wild-type animals underwent no treatment. (A) Electron microscopy showed an increase in number of caveolae compared to control hearts (arrow). (B) and (C) Excised control and hearts subjected to IPC underwent sucrose density fractionation. Protein and cholesterol assays showed increased protein and cholesterol in buoyant fractions (BF) after IPC (*P < 0.05). (D) and (E) Fractions were probed for Cav-1 and Cav-3. Cav-3, but not Cav-1, was increased in buoyant fractions after IPC (representative immunoblots are shown) and confirmed by densitometry normalized to total fraction amounts (E). *P <0.05 relative to respective control. Data are from 6 mice per group.