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. 1989 Jul;27(7):1609–1612. doi: 10.1128/jcm.27.7.1609-1612.1989

Antigen-antibody reaction in solution in capture competition immunoassay for human immunodeficiency virus antibodies.

C M Nielsen 1, B Kvinesdal 1, B F Vestergaard 1
PMCID: PMC267623  PMID: 2768447

Abstract

In the capture competition immunoassay, undiluted serum was reacted in solution with purified human immunodeficiency virus (HIV) antigen in wells of microtest plates coated with anti-HIV immunoglobulin G antibodies (HIV capture antibodies). HIV antibodies present in the serum being tested combined with the HIV antigen and thus blocked (completely or partially) the fixation of the antigen to the capture layer. Unblocked antigenic activity was measured in subsequent steps by the use of biotinylated anti-HIV immunoglobulin G and peroxidase-conjugated avidin. The assay was evaluated in comparison with indirect enzyme-linked immunosorbent assay and Western (immuno-) blot (WB). A total of 180 serum samples which reacted repeatedly as positive in indirect enzyme-linked immunosorbent assay but negative in WB were found to be negative by the capture competition assay. Of 54 serum samples showing dubious reactions (single p24 bands in WB), 53 were clearly separated into positive or negative reactions, whereas 1 serum sample gave a borderline reaction. It was concluded that a characteristic feature of this kind of inhibition assay is a very low frequency of equivocal results.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Biberfeld G., Bredberg-Rådén U., Böttiger B., Putkonen P. O., Blomberg J., Juto P., Wadell G. Blood donor sera with false-positive Western blot reactions to human immunodeficiency virus. Lancet. 1986 Aug 2;2(8501):289–290. doi: 10.1016/s0140-6736(86)92111-2. [DOI] [PubMed] [Google Scholar]
  2. Couroucé A. M., Muller J. Y., Richard D. False-positive Western blot reactions to human immunodeficiency virus in blood donors. Lancet. 1986 Oct 18;2(8512):921–922. doi: 10.1016/s0140-6736(86)90443-5. [DOI] [PubMed] [Google Scholar]
  3. Getchell J. P., Heath J. L., Hicks D. R., Sporborg C., McGrath C. R., Kalyanaraman V. S. Continuous production of a cytopathic human T-lymphotropic virus in a permissive neoplastic T-cell line. J Clin Microbiol. 1986 Apr;23(4):737–742. doi: 10.1128/jcm.23.4.737-742.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kleinman S., Anton-Guirigis H., Culver B. D., Taylor T. H., Prince H. E., Kaplan H. S. Anti-HTLV III ELISA and Western blot testing in a blood donor population: implications for donor notification. Vox Sang. 1986;51(2):143–147. doi: 10.1111/j.1423-0410.1986.tb00231.x. [DOI] [PubMed] [Google Scholar]
  5. Kühnl P., Seidl S., Holzberger G. HLA DR4 antibodies cause positive HTLV-III antibody ELISA results. Lancet. 1985 May 25;1(8439):1222–1223. doi: 10.1016/s0140-6736(85)92910-1. [DOI] [PubMed] [Google Scholar]
  6. Neurath A. R., Strick N., Sproul P., Baker L., Rubinstein P., Stevens C. E., Taylor P., Gallo R. C., Gold J. W., Lee Y. S. Radioimmunoassay and enzyme-linked immunoassay of antibodies to the core protein (P24) of human T-lymphotropic virus (HTLV III). J Virol Methods. 1985 May;11(1):75–86. doi: 10.1016/0166-0934(85)90126-0. [DOI] [PubMed] [Google Scholar]
  7. Nielsen C. M., Bygbjerg I. C., Vestergaard B. F. Detection of HIV antigens in eluates from whole blood collected on filterpaper. Lancet. 1987 Mar 7;1(8532):566–567. doi: 10.1016/s0140-6736(87)90213-3. [DOI] [PubMed] [Google Scholar]
  8. Nielsen C. M., Hansen K., Andersen H. M., Gerstoft J., Vestergaard B. F. A biotin-avidin-amplified inhibition enzyme immunoassay for detection of CMV antibodies in human serum. J Virol Methods. 1987 Jun;16(3):195–208. doi: 10.1016/0166-0934(87)90004-8. [DOI] [PubMed] [Google Scholar]
  9. Pedersen C., Nielsen C. M., Vestergaard B. F., Gerstoft J., Krogsgaard K., Nielsen J. O. Temporal relation of antigenaemia and loss of antibodies to core antigens to development of clinical disease in HIV infection. Br Med J (Clin Res Ed) 1987 Sep 5;295(6598):567–569. doi: 10.1136/bmj.295.6598.567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Reesink H. W., Lelie P. N., Huisman J. G., Schaasberg W., Gonsalves M., Aaij C., Winkel I. N., van der Does J. A., Hekker A. C., Desmyter J. Evaluation of six enzyme immunoassays for antibody against human immunodeficiency virus. Lancet. 1986 Aug 30;2(8505):483–486. doi: 10.1016/s0140-6736(86)90358-2. [DOI] [PubMed] [Google Scholar]
  11. Ward J. W., Grindon A. J., Feorino P. M., Schable C., Parvin M., Allen J. R. Laboratory and epidemiologic evaluation of an enzyme immunoassay for antibodies to HTLV-III. JAMA. 1986 Jul 18;256(3):357–361. [PubMed] [Google Scholar]
  12. Weiss S. H., Mann D. L., Murray C., Popovic M. HLA-DR antibodies and HTLV-III antibody ELISA testing. Lancet. 1985 Jul 20;2(8447):157–157. doi: 10.1016/s0140-6736(85)90261-2. [DOI] [PubMed] [Google Scholar]

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