Abstract
A reproducible method for long-term storage of Campylobacter pylori has been previously described. We cultured 10 strains of C. pylori in brucella broth that was supplemented with 10% fetal bovine serum. After incubation for 24 h, 10% glycerol and an additional 10% fetal bovine serum were added to the cultures, which when then held in storage at -70 degrees C. After 6 months, each of the strains remained viable. This technique, therefore, represents an excellent method for long-term storage of C. pylori isolates.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Blaser M. J. Gastric Campylobacter-like organisms, gastritis, and peptic ulcer disease. Gastroenterology. 1987 Aug;93(2):371–383. doi: 10.1016/0016-5085(87)91028-6. [DOI] [PubMed] [Google Scholar]
- Drumm B., O'Brien A., Cutz E., Sherman P. Campylobacter pyloridis-associated primary gastritis in children. Pediatrics. 1987 Aug;80(2):192–195. [PubMed] [Google Scholar]
- Drumm B., Sherman P., Cutz E., Karmali M. Association of Campylobacter pylori on the gastric mucosa with antral gastritis in children. N Engl J Med. 1987 Jun 18;316(25):1557–1561. doi: 10.1056/NEJM198706183162501. [DOI] [PubMed] [Google Scholar]
- Karmali M. A., Simor A. E., Roscoe M., Fleming P. C., Smith S. S., Lane J. Evaluation of a blood-free, charcoal-based, selective medium for the isolation of Campylobacter organisms from feces. J Clin Microbiol. 1986 Mar;23(3):456–459. doi: 10.1128/jcm.23.3.456-459.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morgan D. R., Freedman R., Depew C. E., Kraft W. G. Growth of Campylobacter pylori in liquid media. J Clin Microbiol. 1987 Nov;25(11):2123–2125. doi: 10.1128/jcm.25.11.2123-2125.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Penner J. L. The genus Campylobacter: a decade of progress. Clin Microbiol Rev. 1988 Apr;1(2):157–172. doi: 10.1128/cmr.1.2.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet. 1983 Jun 4;1(8336):1273–1275. [PubMed] [Google Scholar]