Abstract
The effects of centrifugation of the infection of cell culture with bluetongue virus (BTV) were investigated. Baby hamster kidney cells were infected with BTV with or without centrifugation. Viral antigen was detected by immunofluorescence at 24 h in both centrifuged and noncentrifuged cultures. However, after 24 h of infection, the production of PFU in centrifuged cell cultures was 10- to 20-fold greater than that seen in cultures not centrifuged. In addition, centrifugation enhanced the direct detection of PFU from blood samples collected from a sheep experimentally infected with BTV.
Full text
PDF

Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Crawford S. W., Bowden R. A., Hackman R. C., Gleaves C. A., Meyers J. D., Clark J. G. Rapid detection of cytomegalovirus pulmonary infection by bronchoalveolar lavage and centrifugation culture. Ann Intern Med. 1988 Feb;108(2):180–185. doi: 10.7326/0003-4819-108-2-180. [DOI] [PubMed] [Google Scholar]
- Espy M. J., Smith T. F. Detection of herpes simplex virus in conventional tube cell cultures and in shell vials with a DNA probe kit and monoclonal antibodies. J Clin Microbiol. 1988 Jan;26(1):22–24. doi: 10.1128/jcm.26.1.22-24.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hudson J. B. Further studies on the mechanism of centrifugal enhancement of cytomegalovirus infectivity. J Virol Methods. 1988 Feb;19(2):97–108. doi: 10.1016/0166-0934(88)90153-x. [DOI] [PubMed] [Google Scholar]
- Hudson J. B., Misra V., Mosmann T. R. Cytomegalovirus infectivity: analysis of the phenomenon of centrifugal enhancement of infectivity. Virology. 1976 Jul 1;72(1):235–243. doi: 10.1016/0042-6822(76)90326-3. [DOI] [PubMed] [Google Scholar]
- Jochim M. M. An overview of diagnostics for bluetongue. Prog Clin Biol Res. 1985;178:423–433. [PubMed] [Google Scholar]
- Luedke A. J., Erasmus B. J., Sawyer M., Goldsmit L., St George T. D., Metcalf H. E., Stott J. L., Pearson J. E., Taylor W. P. WHO/FAO Working Team Report: virology. Prog Clin Biol Res. 1985;178:665–688. [PubMed] [Google Scholar]
- Martin W. J., 2nd, Smith T. F. Rapid detection of cytomegalovirus in bronchoalveolar lavage specimens by a monoclonal antibody method. J Clin Microbiol. 1986 Jun;23(6):1006–1008. doi: 10.1128/jcm.23.6.1006-1008.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Verwoerd D. W., Els H. J., De Villiers E. M., Huismans H. Structure of the bluetongue virus capsid. J Virol. 1972 Oct;10(4):783–794. doi: 10.1128/jvi.10.4.783-794.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Verwoerd D. W., Louw H., Oellermann R. A. Characterization of bluetongue virus ribonucleic acid. J Virol. 1970 Jan;5(1):1–7. doi: 10.1128/jvi.5.1.1-7.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]