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. 1989 Jul;27(7):1661–1663. doi: 10.1128/jcm.27.7.1661-1663.1989

Laboratory diagnosis of pulmonary toxoplasmosis in patients with acquired immunodeficiency syndrome.

F Derouin 1, C Sarfati 1, B Beauvais 1, M C Iliou 1, L Dehen 1, M Lariviere 1
PMCID: PMC267636  PMID: 2671023

Abstract

In four cases of pulmonary toxoplasmosis occurring in patients with acquired immunodeficiency syndrome, Toxoplasma sp. was discovered in bronchoalveolar-lavage fluid (three cases) and in lung biopsy specimen (one case) by using the following methods: direct examination of smears stained with eosine-methylene blue fast stain, indirect immunofluorescence assay, and inoculation of MRC5 fibroblast cell line in tissue culture.

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Selected References

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  1. Catterall J. R., Hofflin J. M., Remington J. S. Pulmonary toxoplasmosis. Am Rev Respir Dis. 1986 Apr;133(4):704–705. doi: 10.1164/arrd.1986.133.4.704. [DOI] [PubMed] [Google Scholar]
  2. Derouin F., Mazeron M. C., Garin Y. J. Comparative study of tissue culture and mouse inoculation methods for demonstration of Toxoplasma gondii. J Clin Microbiol. 1987 Sep;25(9):1597–1600. doi: 10.1128/jcm.25.9.1597-1600.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Desmonts G., Naot Y., Remington J. S. Immunoglobulin M-immunosorbent agglutination assay for diagnosis of infectious diseases: diagnosis of acute congenital and acquired Toxoplasma infections. J Clin Microbiol. 1981 Nov;14(5):486–491. doi: 10.1128/jcm.14.5.486-491.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gill V. J., Nelson N. A., Stock F., Evans G. Optimal use of the cytocentrifuge for recovery and diagnosis of Pneumocystis carinii in bronchoalveolar lavage and sputum specimens. J Clin Microbiol. 1988 Sep;26(9):1641–1644. doi: 10.1128/jcm.26.9.1641-1644.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Luft B. J., Remington J. S. AIDS commentary. Toxoplasmic encephalitis. J Infect Dis. 1988 Jan;157(1):1–6. doi: 10.1093/infdis/157.1.1. [DOI] [PubMed] [Google Scholar]
  6. Mendelson M. H., Finkel L. J., Meyers B. R., Lieberman J. P., Hirschman S. Z. Pulmonary toxoplasmosis in AIDS. Scand J Infect Dis. 1987;19(6):703–706. doi: 10.3109/00365548709117209. [DOI] [PubMed] [Google Scholar]
  7. Murray J. F., Felton C. P., Garay S. M., Gottlieb M. S., Hopewell P. C., Stover D. E., Teirstein A. S. Pulmonary complications of the acquired immunodeficiency syndrome. Report of a National Heart, Lung, and Blood Institute workshop. N Engl J Med. 1984 Jun 21;310(25):1682–1688. doi: 10.1056/NEJM198406213102529. [DOI] [PubMed] [Google Scholar]
  8. Niel G., Desmonts G., Gentilini M. Immunofluorescence quantitative et diagnostic sérologique de la toxoplasmose: introduction des unités internationales dans l'expression des positivés. Pathol Biol (Paris) 1973 Feb;21(2):157–161. [PubMed] [Google Scholar]
  9. Pintozzi R. L. Modified Grocott's methenamine silver nitrate method for quick staining of Pneumocystis carinii. J Clin Pathol. 1978 Aug;31(8):803–805. doi: 10.1136/jcp.31.8.803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Touboul J. L., Salmon D., Lancastre F., Mayaud C., Fermand J. P., Fouret P., Akoun G. Pneumopathie à Toxoplasma gondii chez un patient atteint de syndrome d'immunodépression acquis: mise en évidence du parasite par lavage bronchiolo-alvéolaire. Rev Pneumol Clin. 1986;42(3):150–152. [PubMed] [Google Scholar]
  11. Tourani J. M., Israël-Biet D., Venet A., Andrieu J. M. Unusual pulmonary infection in a puzzling presentation of AIDS. Lancet. 1985 Apr 27;1(8435):989–989. doi: 10.1016/s0140-6736(85)91769-6. [DOI] [PubMed] [Google Scholar]

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