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. 1989 Jul;27(7):1684–1688. doi: 10.1128/jcm.27.7.1684-1688.1989

Simultaneous detection of Escherichia coli heat-stable and heat-labile enterotoxin genes with a single RNA probe.

X Sáez-Llorens 1, L M Guzman-Verduzco 1, S Shelton 1, J D Nelson 1, Y M Kupersztoch 1
PMCID: PMC267644  PMID: 2671025

Abstract

A single RNA probe was synthesized and used to detect simultaneously the methanol-soluble heat-stable enterotoxin and heat-labile enterotoxin genes in Escherichia coli strains. The results with the biotinylated or radioactive probe correlated 100% with the biological assay results for both toxins. The RNA probe detected the three known heat-stable enterotoxin A alleles.

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Selected References

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