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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1989 Jul;27(7):1695–1696. doi: 10.1128/jcm.27.7.1695-1696.1989

Detection of herpes simplex virus by using A549 cells in centrifugation culture with a rapid membrane enzyme immunoassay.

D A Hursh 1, S F Wendt 1, C F Lee 1, C A Gleaves 1
PMCID: PMC267647  PMID: 2549094

Abstract

The RAMP herpes simplex virus (HSV) culture confirmation test was compared with immunofluorescence (IF) staining with a specific HSV monoclonal antibody reagent for the detection of HSV in centrifugation culture. The RAMP test detected 47 of 57 IF-positive specimens (sensitivity, 88.6%) and agreed with 217 of 220 IF-negative specimens (specificity, 98.6%). The RAMP test can be performed in less than 15 min and gives an immediate visual result. However, the sensitivity and the false-positive and false-negative results need further investigation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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