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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1989 Jul;27(7):1697–1699. doi: 10.1128/jcm.27.7.1697-1699.1989

Identification of Escherichia coli that produces heat-stable enterotoxin STA by a commercially available enzyme-linked immunoassay and comparison of the assay with infant mouse and DNA probe tests.

S M Scotland 1, G A Willshaw 1, B Said 1, H R Smith 1, B Rowe 1
PMCID: PMC267648  PMID: 2671027

Abstract

By using the infant mouse test and a commercially available competitive enzyme-linked immunosorbent assay (ELISA), 100 strains of Escherichia coli carrying STA1 or STA2 genes were shown to produce the heat-stable enterotoxin STA. An additional 100 strains were negative in both tests. The ELISA was easy to perform, and results were available within 24 h. Testing strains with an enzyme-linked DNA probe kit that incorporated both STA1- and STA2-specific oligonucleotides showed that the 100 strains positive in the mouse test and ELISA also hybridized with the mixed probe. Two strains carrying STA1 genes but negative in the mouse test and ELISA also hybridized with the mixed probe.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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