Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1989 Aug;27(8):1863–1866. doi: 10.1128/jcm.27.8.1863-1866.1989

Cytobrush in collection of cervical specimens for detection of Chlamydia trachomatis.

J Moncada 1, J Schachter 1, M Shipp 1, G Bolan 1, J Wilber 1
PMCID: PMC267685  PMID: 2671035

Abstract

Methods of collecting endocervical samples for the detection of Chlamydia trachomatis were evaluated. We compared Calgiswab and cytobrush for isolation in cell culture and Dacron swab and cytobrush for direct fluorescent-antibody (DFA) testing for 632 females attending a sexually transmitted disease clinic. An additional specimen for enzyme immunoassay (EIA) was also collected. True-positives were identified as tissue culture positive and/or both DFA and EIA positive. Use of the cytobrush significantly improved the sensitivity of both the culture (69% with swab and 100% with cytobrush) and DFA testing (68% with swab and 85% with cytobrush). The EIA sensitivity was 85%. The specificity of each test was greater than or equal to 98%. The cytobrush appears to be the superior method for the collection of cervical samples from nonpregnant women.

Full text

PDF
1863

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chernesky M. A., Mahony J. B., Castriciano S., Mores M., Stewart I. O., Landis S. J., Seidelman W., Sargeant E. J., Leman C. Detection of Chlamydia trachomatis antigens by enzyme immunoassay and immunofluorescence in genital specimens from symptomatic and asymptomatic men and women. J Infect Dis. 1986 Jul;154(1):141–148. doi: 10.1093/infdis/154.1.141. [DOI] [PubMed] [Google Scholar]
  2. Ciotti R. A., Sondheimer S. J., Nachamkin I. Detecting Chlamydia trachomatis by direct immunofluorescence using a Cytobrush sampling technique. Genitourin Med. 1988 Aug;64(4):245–246. doi: 10.1136/sti.64.4.245. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Forbes B. A., Bartholoma N., McMillan J., Roefaro M., Weiner L., Welych L. Evaluation of a monoclonal antibody test to detect chlamydia in cervical and urethral specimens. J Clin Microbiol. 1986 Jun;23(6):1136–1137. doi: 10.1128/jcm.23.6.1136-1137.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Judson B. A., Lambert P. P. Improved Syva MicroTrak Chlamydia trachomatis direct test method. J Clin Microbiol. 1988 Dec;26(12):2657–2658. doi: 10.1128/jcm.26.12.2657-2658.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lefebvre J., Laperrière H., Rousseau H., Massé R. Comparison of three techniques for detection of Chlamydia trachomatis in endocervical specimens from asymptomatic women. J Clin Microbiol. 1988 Apr;26(4):726–731. doi: 10.1128/jcm.26.4.726-731.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lindner L. E., Nettum J. A., Miller S. L., Altman K. H. Comparison of scrape, swab, and cytobrush samples for the diagnosis of cervical chlamydial infection by immunofluorescence. Diagn Microbiol Infect Dis. 1987 Nov;8(3):179–182. doi: 10.1016/0732-8893(87)90169-6. [DOI] [PubMed] [Google Scholar]
  7. Lipkin E. S., Moncada J. V., Shafer M. A., Wilson T. E., Schachter J. Comparison of monoclonal antibody staining and culture in diagnosing cervical chlamydial infection. J Clin Microbiol. 1986 Jan;23(1):114–117. doi: 10.1128/jcm.23.1.114-117.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mårdh P. A., Zeeberg B. Toxic effect of sampling swabs and transportation test tubes on the formation of intracytoplasmic inclusions of Chlamydia trachomatis in McCoy cell cultures. Br J Vener Dis. 1981 Aug;57(4):268–272. doi: 10.1136/sti.57.4.268. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Paavonen J. Chlamydia trachomatis-induced urethritis in female partners of men with nongonococcal urethritis. Sex Transm Dis. 1979 Apr-Jun;6(2):69–71. doi: 10.1097/00007435-197904000-00005. [DOI] [PubMed] [Google Scholar]
  10. Ripa K. T., Mårdh P. A. Cultivation of Chlamydia trachomatis in cycloheximide-treated mccoy cells. J Clin Microbiol. 1977 Oct;6(4):328–331. doi: 10.1128/jcm.6.4.328-331.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schachter J. Immunodiagnosis of sexually transmitted disease. Yale J Biol Med. 1985 Sep-Oct;58(5):443–452. [PMC free article] [PubMed] [Google Scholar]
  12. Schachter J., Martin D. H. Failure of multiple passages to increase chlamydial recovery. J Clin Microbiol. 1987 Oct;25(10):1851–1853. doi: 10.1128/jcm.25.10.1851-1853.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schachter J., Moncada J., Dawson C. R., Sheppard J., Courtright P., Said M. E., Zaki S., Hafez S. F., Lorincz A. Nonculture methods for diagnosing chlamydial infection in patients with trachoma: a clue to the pathogenesis of the disease? J Infect Dis. 1988 Dec;158(6):1347–1352. doi: 10.1093/infdis/158.6.1347. [DOI] [PubMed] [Google Scholar]
  14. Schachter J. Rapid diagnosis of sexually transmitted diseases--speed has a price. Diagn Microbiol Infect Dis. 1986 Mar;4(3):185–189. doi: 10.1016/0732-8893(86)90097-0. [DOI] [PubMed] [Google Scholar]
  15. Schachter J., Stoner E., Moncada J. Screening for chlamydial infections in women attending family planning clinics. West J Med. 1983 Mar;138(3):375–379. [PMC free article] [PubMed] [Google Scholar]
  16. Smith J. W., Rogers R. E., Katz B. P., Brickler J. F., Lineback P. L., Van der Pol B., Jones R. B. Diagnosis of chlamydial infection in women attending antenatal and gynecologic clinics. J Clin Microbiol. 1987 May;25(5):868–872. doi: 10.1128/jcm.25.5.868-872.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Thomas B. J., Evans R. T., Hawkins D. A., Taylor-Robinson D. Sensitivity of detecting Chlamydia trachomatis elementary bodies in smears by use of a fluorescein labelled monoclonal antibody: comparison with conventional chlamydial isolation. J Clin Pathol. 1984 Jul;37(7):812–816. doi: 10.1136/jcp.37.7.812. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Washington A. E., Johnson R. E., Sanders L. L., Jr Chlamydia trachomatis infections in the United States. What are they costing us? JAMA. 1987 Apr 17;257(15):2070–2072. [PubMed] [Google Scholar]
  19. Weiland T. L., Noller K. L., Smith T. F., Ory S. J. Comparison of Dacron-tipped applicator and cytobrush for detection of chlamydial infections. J Clin Microbiol. 1988 Nov;26(11):2437–2438. doi: 10.1128/jcm.26.11.2437-2438.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES