Abstract
Early detection of sexually transmitted human immunodeficiency virus type 1 (HIV-1) infection was investigated in newly infected persons to determine the sensitivities of currently available serologic techniques. Serial serum samples were obtained from 51 newly infected persons in a cohort of 1,153 homosexual or bisexual men participating in the Baltimore Center of the Multi-Center AIDS Cohort Study during the first 2.5 years of follow-up. Of 51 participants, 45 seroconverted between any two seminannual visits and 6 were found to have been infected just prior to study entry. Five enzyme-linked immunosorbent assays (ELISAs), two immunoblots, and an HIV-1 P24 antigen capture assay were performed on a panel of all serial serum samples from these individuals. The sensitivity of ELISAs varied between 50 and 84% in seroconverters with less-developed antibody response. In this group of seroconverters, the most sensitive antibody assay was an immunoblot from Biotech (95%) and HIV-1 P24 was found infrequently (9.5%). The sensitivities of ELISAs and immunoblot were 100% in individuals with more-developed antibody patterns, and no HIV-1 P24 was detected among them.
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- Esteban J. I., Shih J. W., Tai C. C., Bodner A. J., Kay J. W., Alter H. J. Importance of western blot analysis in predicting infectivity of anti-HTLV-III/LAV positive blood. Lancet. 1985 Nov 16;2(8464):1083–1086. doi: 10.1016/s0140-6736(85)90683-x. [DOI] [PubMed] [Google Scholar]
- Farzadegan H., Polis M. A., Wolinsky S. M., Rinaldo C. R., Jr, Sninsky J. J., Kwok S., Griffith R. L., Kaslow R. A., Phair J. P., Polk B. F. Loss of human immunodeficiency virus type 1 (HIV-1) antibodies with evidence of viral infection in asymptomatic homosexual men. A report from the Multicenter AIDS Cohort Study. Ann Intern Med. 1988 Jun;108(6):785–790. doi: 10.7326/0003-4819-108-6-785. [DOI] [PubMed] [Google Scholar]
- Gaines H., von Sydow M., Sönnerborg A., Albert J., Czajkowski J., Pehrson P. O., Chiodi F., Moberg L., Fenyö E. M., Asjö B. Antibody response in primary human immunodeficiency virus infection. Lancet. 1987 May 30;1(8544):1249–1253. doi: 10.1016/s0140-6736(87)92696-1. [DOI] [PubMed] [Google Scholar]
- Gallo R. C., Salahuddin S. Z., Popovic M., Shearer G. M., Kaplan M., Haynes B. F., Palker T. J., Redfield R., Oleske J., Safai B. Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS. Science. 1984 May 4;224(4648):500–503. doi: 10.1126/science.6200936. [DOI] [PubMed] [Google Scholar]
- Grillner L., Landqvist M., Johansson M., Schulman S. False negative result by the wellcozyme anti-HIV assay in testing an HIV positive haemophiliac. Lancet. 1987 May 23;1(8543):1200–1201. doi: 10.1016/s0140-6736(87)92165-9. [DOI] [PubMed] [Google Scholar]
- Marlink R. G., Allan J. S., McLane M. F., Essex M., Anderson K. C., Groopman J. E. Low sensitivity of ELISA testing in early HIV infection. N Engl J Med. 1986 Dec 11;315(24):1549–1549. doi: 10.1056/NEJM198612113152413. [DOI] [PubMed] [Google Scholar]
- Ozanne G., Fauvel M. Performance and reliability of five commercial enzyme-linked immunosorbent assay kits in screening for anti-human immunodeficiency virus antibody in high-risk subjects. J Clin Microbiol. 1988 Aug;26(8):1496–1500. doi: 10.1128/jcm.26.8.1496-1500.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Saah A. J., Farzadegan H., Fox R., Nishanian P., Rinaldo C. R., Jr, Phair J. P., Fahey J. L., Lee T. H., Polk B. F. Detection of early antibodies in human immunodeficiency virus infection by enzyme-linked immunosorbent assay, Western blot, and radioimmunoprecipitation. J Clin Microbiol. 1987 Sep;25(9):1605–1610. doi: 10.1128/jcm.25.9.1605-1610.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ulstrup J. C., Skaug K., Figenschau K. J., Orstavik I., Bruun J. N., Petersen G. Sensitivity of western blotting (compared with ELISA and immunofluorescence) during seroconversion after HTLV-III infection. Lancet. 1986 May 17;1(8490):1151–1152. doi: 10.1016/s0140-6736(86)91862-3. [DOI] [PubMed] [Google Scholar]