Abstract
Screening leukocytes for cytomegalovirus (CMV) by shell vial assay gave unsatisfactory results. Of 10 positive specimens (114 samples), only 2 showing CMV could be detected. Disruption of leukocytes prior to their use in shell vial assays increased the sensitivity of CMV detection considerably: of 32 leukocyte specimens from transplant patients with signs of CMV disease, 13 were positive with disruption and only 3 were positive without. With this modification, 17 transplant patients with suspected CMV infection were regularly screened. Viremia could be detected in 10 cases by the shell vial assay and in 11 cases by the direct detection of immediate early antigen. On the average, viremia was detected 11 days before immunoglobulin M or typical clinical symptoms.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cheeseman S. H., Rubin R. H., Stewart J. A., Tolkoff-Rubin N. E., Cosimi A. B., Cantell K., Gilbert J., Winkle S., Herrin J. T., Black P. H. Controlled clinical trial of prophylactic human-leukocyte interferon in renal transplantation. Effects on cytomegalovirus and herpes simplex virus infections. N Engl J Med. 1979 Jun 14;300(24):1345–1349. doi: 10.1056/NEJM197906143002401. [DOI] [PubMed] [Google Scholar]
- Griffiths P. D., Panjwani D. D., Stirk P. R., Ball M. G., Ganczakowski M., Blacklock H. A., Prentice H. G. Rapid diagnosis of cytomegalovirus infection in immunocompromised patients by detection of early antigen fluorescent foci. Lancet. 1984 Dec 1;2(8414):1242–1245. doi: 10.1016/s0140-6736(84)92797-1. [DOI] [PubMed] [Google Scholar]
- Meyers J. D., Flournoy N., Thomas E. D. Risk factors for cytomegalovirus infection after human marrow transplantation. J Infect Dis. 1986 Mar;153(3):478–488. doi: 10.1093/infdis/153.3.478. [DOI] [PubMed] [Google Scholar]
- Paya C. V., Wold A. D., Ilstrup D. M., Smith T. F. Evaluation of number of shell vial cell cultures per clinical specimen for rapid diagnosis of cytomegalovirus infection. J Clin Microbiol. 1988 Feb;26(2):198–200. doi: 10.1128/jcm.26.2.198-200.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rubin R. H., Cosimi A. B., Hirsch M. S., Herrin J. T., Russell P. S., Tolkoff-Rubin N. E. Effects of antithymocyte globulin on cytomegalovirus infection in renal transplant recipients. Transplantation. 1981 Feb;31(2):143–145. doi: 10.1097/00007890-198102000-00016. [DOI] [PubMed] [Google Scholar]
- Shuster E. A., Beneke J. S., Tegtmeier G. E., Pearson G. R., Gleaves C. A., Wold A. D., Smith T. F. Monoclonal antibody for rapid laboratory detection of cytomegalovirus infections: characterization and diagnostic application. Mayo Clin Proc. 1985 Sep;60(9):577–585. doi: 10.1016/s0025-6196(12)60979-3. [DOI] [PubMed] [Google Scholar]
- van der Bij W., Torensma R., van Son W. J., Anema J., Schirm J., Tegzess A. M., The T. H. Rapid immunodiagnosis of active cytomegalovirus infection by monoclonal antibody staining of blood leucocytes. J Med Virol. 1988 Jun;25(2):179–188. doi: 10.1002/jmv.1890250208. [DOI] [PubMed] [Google Scholar]