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. 1989 Sep;27(9):1946–1948. doi: 10.1128/jcm.27.9.1946-1948.1989

Production of a toxic shock syndrome toxin variant by Staphylococcus aureus strains associated with sheep, goats, and cows.

G Ho 1, W H Campbell 1, M S Bergdoll 1, E Carlson 1
PMCID: PMC267714  PMID: 2778060

Abstract

A toxic shock syndrome toxin (TSST) variant with an isoelectric point (pI) of 8.6 produced by an ovine-associated Staphylococcus aureus strain was described previously. Analysis of additional strains associated with sheep, goats, cows, and humans by isoelectric focusing with immunoblotting using monoclonal antibodies revealed that all 18 strains associated with sheep and all 12 strains associated with goats produced the TSST variant. Only 1 of 10 bovine-associated strains and no human-associated strains produced the variant, whereas the others produced TSST-1 (pI between 7.0 and 7.2). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis with immunoblotting indicated that both TSST-1 and the TSST variant had a molecular size of 24 kilodaltons.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avena R. M., Bergdoll M. S. Purification and some physicochemical properties of enterotoxin C, Staphylococcus aureus strain 361. Biochemistry. 1967 May;6(5):1474–1480. doi: 10.1021/bi00857a033. [DOI] [PubMed] [Google Scholar]
  2. Bergdoll M. S., Crass B. A., Reiser R. F., Robbins R. N., Davis J. P. A new staphylococcal enterotoxin, enterotoxin F, associated with toxic-shock-syndrome Staphylococcus aureus isolates. Lancet. 1981 May 9;1(8228):1017–1021. doi: 10.1016/s0140-6736(81)92186-3. [DOI] [PubMed] [Google Scholar]
  3. Blomster-Hautamaa D. A., Kreiswirth B. N., Novick R. P., Schlievert P. M. Resolution of highly purified toxic-shock syndrome toxin 1 into two distinct proteins by isoelectric focusing. Biochemistry. 1986 Jan 14;25(1):54–59. doi: 10.1021/bi00349a009. [DOI] [PubMed] [Google Scholar]
  4. Bonventre P. F., Thompson M. R., Adinolfi L. E., Gillis Z. A., Parsonnet J. Neutralization of toxic shock syndrome toxin-1 by monoclonal antibodies in vitro and in vivo. Infect Immun. 1988 Jan;56(1):135–141. doi: 10.1128/iai.56.1.135-141.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Change P. C., Yano Y., Dighton M., Dickie N. Fractionation of staphylococcal enterotoxin C 2 by isoelectric focusing. Can J Microbiol. 1971 Nov;17(11):1367–1372. doi: 10.1139/m71-218. [DOI] [PubMed] [Google Scholar]
  6. Goding J. W. Conjugation of antibodies with fluorochromes: modifications to the standard methods. J Immunol Methods. 1976;13(3-4):215–226. doi: 10.1016/0022-1759(76)90068-5. [DOI] [PubMed] [Google Scholar]
  7. Hawkes R., Niday E., Gordon J. A dot-immunobinding assay for monoclonal and other antibodies. Anal Biochem. 1982 Jan 1;119(1):142–147. doi: 10.1016/0003-2697(82)90677-7. [DOI] [PubMed] [Google Scholar]
  8. Ho G., Campbell W. H., Carlson E. Ovine-associated Staphylococcus aureus protein with immunochemical similarity to toxic shock syndrome toxin 1. J Clin Microbiol. 1989 Jan;27(1):210–212. doi: 10.1128/jcm.27.1.210-212.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Reiser R. F., Robbins R. N., Khoe G. P., Bergdoll M. S. Purification and some physicochemical properties of toxic-shock toxin. Biochemistry. 1983 Aug 2;22(16):3907–3912. doi: 10.1021/bi00285a028. [DOI] [PubMed] [Google Scholar]
  11. Schantz E. J., Roessler W. G., Woodburn M. J., Lynch J. M., Jacoby H. M., Silverman S. J., Gorman J. C., Spero L. Purification and some chemical and physical properties of staphylococcal enterotoxin A. Biochemistry. 1972 Feb 1;11(3):360–366. doi: 10.1021/bi00753a009. [DOI] [PubMed] [Google Scholar]
  12. Schlievert P. M., Shands K. N., Dan B. B., Schmid G. P., Nishimura R. D. Identification and characterization of an exotoxin from Staphylococcus aureus associated with toxic-shock syndrome. J Infect Dis. 1981 Apr;143(4):509–516. doi: 10.1093/infdis/143.4.509. [DOI] [PubMed] [Google Scholar]
  13. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]

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