Abstract
Verotoxin (VT), which is immunologically unrelated to VT1 (Shiga-like toxin I), was purified from the culture filtrate of Escherichia coli hemorrhagic colitis serogroup O157:H7 strain 3657 by copper ion chelate affinity chromatography followed by anion-exchange chromatography. The isoelectric point by sucrose density gradient isoelectric focusing was 5.0, the molecular weight by gel filtration on Superose 12 was about 60,000, and the 50% cytopathic dose for Vero cells was about 1 pg. This toxin was found by immunological methods to be the predominant VT in E. coli O157 isolates associated with illness in North America, with 38 of 42 strains tested producing this toxin, 20 in combination with VT1. VT from strain 3657 is immunologically identical to the described Shiga-like toxin II (VT2) of E. coli strains (from the United States) K-12(pEB1) and C600(933W) but only partially related to VT of strain E32511 (from the United Kingdom), the first to be named VT2.
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Selected References
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- Bopp C. A., Greene K. D., Downes F. P., Sowers E. G., Wells J. G., Wachsmuth I. K. Unusual verotoxin-producing Escherichia coli associated with hemorrhagic colitis. J Clin Microbiol. 1987 Aug;25(8):1486–1489. doi: 10.1128/jcm.25.8.1486-1489.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Downes F. P., Barrett T. J., Green J. H., Aloisio C. H., Spika J. S., Strockbine N. A., Wachsmuth I. K. Affinity purification and characterization of Shiga-like toxin II and production of toxin-specific monoclonal antibodies. Infect Immun. 1988 Aug;56(8):1926–1933. doi: 10.1128/iai.56.8.1926-1933.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Karmali M. A., Petric M., Lim C., Fleming P. C., Arbus G. S., Lior H. The association between idiopathic hemolytic uremic syndrome and infection by verotoxin-producing Escherichia coli. J Infect Dis. 1985 May;151(5):775–782. doi: 10.1093/infdis/151.5.775. [DOI] [PubMed] [Google Scholar]
- Konowalchuk J., Dickie N., Stavric S., Speirs J. I. Comparative studies of five heat-labile toxic products of Escherichia coli. Infect Immun. 1978 Dec;22(3):644–648. doi: 10.1128/iai.22.3.644-648.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Konowalchuk J., Dickie N., Stavric S., Speirs J. I. Properties of an Escherichia coli cytotoxin. Infect Immun. 1978 May;20(2):575–577. doi: 10.1128/iai.20.2.575-577.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Konowalchuk J., Speirs J. I., Stavric S. Vero response to a cytotoxin of Escherichia coli. Infect Immun. 1977 Dec;18(3):775–779. doi: 10.1128/iai.18.3.775-779.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Marques L. R., Moore M. A., Wells J. G., Wachsmuth I. K., O'Brien A. D. Production of Shiga-like toxin by Escherichia coli. J Infect Dis. 1986 Aug;154(2):338–341. doi: 10.1093/infdis/154.2.338. [DOI] [PubMed] [Google Scholar]
- Noda M., Yutsudo T., Nakabayashi N., Hirayama T., Takeda Y. Purification and some properties of Shiga-like toxin from Escherichia coli O157:H7 that is immunologically identical to Shiga toxin. Microb Pathog. 1987 May;2(5):339–349. doi: 10.1016/0882-4010(87)90076-3. [DOI] [PubMed] [Google Scholar]
- O'Brien A. D., LaVeck G. D. Purification and characterization of a Shigella dysenteriae 1-like toxin produced by Escherichia coli. Infect Immun. 1983 May;40(2):675–683. doi: 10.1128/iai.40.2.675-683.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'Brien A. D., LaVeck G. D., Thompson M. R., Formal S. B. Production of Shigella dysenteriae type 1-like cytotoxin by Escherichia coli. J Infect Dis. 1982 Dec;146(6):763–769. doi: 10.1093/infdis/146.6.763. [DOI] [PubMed] [Google Scholar]
- Padhye V. V., Beery J. T., Kittell F. B., Doyle M. P. Colonic hemorrhage produced in mice by a unique vero cell cytotoxin from an Escherichia coli strain that causes hemorrhagic colitis. J Infect Dis. 1987 Jun;155(6):1249–1253. doi: 10.1093/infdis/155.6.1249. [DOI] [PubMed] [Google Scholar]
- Padhye V. V., Kittell F. B., Doyle M. P. Purification and physicochemical properties of a unique Vero cell cytotoxin from Escherichia coli O157:H7. Biochem Biophys Res Commun. 1986 Sep 14;139(2):424–430. doi: 10.1016/s0006-291x(86)80008-0. [DOI] [PubMed] [Google Scholar]
- Pai C. H., Gordon R., Sims H. V., Bryan L. E. Sporadic cases of hemorrhagic colitis associated with Escherichia coli O157:H7. Clinical, epidemiologic, and bacteriologic features. Ann Intern Med. 1984 Dec;101(6):738–742. doi: 10.7326/0003-4819-101-6-738. [DOI] [PubMed] [Google Scholar]
- Riley L. W., Remis R. S., Helgerson S. D., McGee H. B., Wells J. G., Davis B. R., Hebert R. J., Olcott E. S., Johnson L. M., Hargrett N. T. Hemorrhagic colitis associated with a rare Escherichia coli serotype. N Engl J Med. 1983 Mar 24;308(12):681–685. doi: 10.1056/NEJM198303243081203. [DOI] [PubMed] [Google Scholar]
- Riley L. W. The epidemiologic, clinical, and microbiologic features of hemorrhagic colitis. Annu Rev Microbiol. 1987;41:383–407. doi: 10.1146/annurev.mi.41.100187.002123. [DOI] [PubMed] [Google Scholar]
- Ryan C. A., Tauxe R. V., Hosek G. W., Wells J. G., Stoesz P. A., McFadden H. W., Jr, Smith P. W., Wright G. F., Blake P. A. Escherichia coli O157:H7 diarrhea in a nursing home: clinical, epidemiological, and pathological findings. J Infect Dis. 1986 Oct;154(4):631–638. doi: 10.1093/infdis/154.4.631. [DOI] [PubMed] [Google Scholar]
- Strockbine N. A., Marques L. R., Holmes R. K., O'Brien A. D. Characterization of monoclonal antibodies against Shiga-like toxin from Escherichia coli. Infect Immun. 1985 Dec;50(3):695–700. doi: 10.1128/iai.50.3.695-700.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Strockbine N. A., Marques L. R., Newland J. W., Smith H. W., Holmes R. K., O'Brien A. D. Two toxin-converting phages from Escherichia coli O157:H7 strain 933 encode antigenically distinct toxins with similar biologic activities. Infect Immun. 1986 Jul;53(1):135–140. doi: 10.1128/iai.53.1.135-140.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wells J. G., Davis B. R., Wachsmuth I. K., Riley L. W., Remis R. S., Sokolow R., Morris G. K. Laboratory investigation of hemorrhagic colitis outbreaks associated with a rare Escherichia coli serotype. J Clin Microbiol. 1983 Sep;18(3):512–520. doi: 10.1128/jcm.18.3.512-520.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yutsudo T., Nakabayashi N., Hirayama T., Takeda Y. Purification and some properties of a Vero toxin from Escherichia coli O157:H7 that is immunologically unrelated to Shiga toxin. Microb Pathog. 1987 Jul;3(1):21–30. doi: 10.1016/0882-4010(87)90034-9. [DOI] [PubMed] [Google Scholar]