Abstract
A quantitative method to determine glycocalyx production by strains of viridans group streptococci from patients with endocarditis is presented. There is good correlation between this new tryptophan quantitative assay and qualitative assays employing polysaccharide stains (ruthenium red, periodic acid-Schiff, and Cellufluor) or the Molisch test. The quantification of the glycocalyx production in glucose substrate in vitro by viridans group streptococci correlates with the size of cardiac vegetation and ease of antimicrobial sterilization in experimental endocarditis. The relationship of in vitro quantification of glycocalyx to maintenance of infection, morbidity of infection, and antimicrobial treatment is discussed.
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- Baltimore R. S., Mitchell M. Immunologic investigations of mucoid strains of Pseudomonas aeruginosa: comparison of susceptibility to opsonic antibody in mucoid and nonmucoid strains. J Infect Dis. 1980 Feb;141(2):238–247. doi: 10.1093/infdis/141.2.238. [DOI] [PubMed] [Google Scholar]
- Dall L., Barnes W. G., Lane J. W., Mills J. Enzymatic modification of glycocalyx in the treatment of experimental endocarditis due to viridans streptococci. J Infect Dis. 1987 Nov;156(5):736–740. doi: 10.1093/infdis/156.5.736. [DOI] [PubMed] [Google Scholar]
- Durack D. T., Beeson P. B. Experimental bacterial endocarditis. I. Colonization of a sterile vegetation. Br J Exp Pathol. 1972 Feb;53(1):44–49. [PMC free article] [PubMed] [Google Scholar]
- Gould K., Ramirez-Ronda C. H., Holmes R. K., Sanford J. P. Adherence of bacteria to heart valves in vitro. J Clin Invest. 1975 Dec;56(6):1364–1370. doi: 10.1172/JCI108216. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Govan J. R., Fyfe J. A. Mucoid Pseudomonas aeruginosa and cystic fibrosis: resistance of the mucoid from to carbenicillin, flucloxacillin and tobramycin and the isolation of mucoid variants in vitro. J Antimicrob Chemother. 1978 May;4(3):233–240. doi: 10.1093/jac/4.3.233. [DOI] [PubMed] [Google Scholar]
- Johnson G. M., Lee D. A., Regelmann W. E., Gray E. D., Peters G., Quie P. G. Interference with granulocyte function by Staphylococcus epidermidis slime. Infect Immun. 1986 Oct;54(1):13–20. doi: 10.1128/iai.54.1.13-20.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mills J., Pulliam L., Dall L., Marzouk J., Wilson W., Costerton J. W. Exopolysaccharide production by viridans streptococci in experimental endocarditis. Infect Immun. 1984 Jan;43(1):359–367. doi: 10.1128/iai.43.1.359-367.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Perlman B. B., Freedman L. R. Experimental endocarditis. II. Staphylococcal infection of the aortic valve following placement of a polyethylene catheter in the left side of the heart. Yale J Biol Med. 1971 Oct;44(2):206–213. [PMC free article] [PubMed] [Google Scholar]
- Pulliam L., Dall L., Inokuchi S., Wilson W., Hadley W. K., Mills J. Effects of exopolysaccharide production by viridans streptococci on penicillin therapy of experimental endocarditis. J Infect Dis. 1985 Jan;151(1):153–156. doi: 10.1093/infdis/151.1.153. [DOI] [PubMed] [Google Scholar]
- Pulliam L., Porschen R. K., Hadley W. K. Biochemical properties of CO2-dependent streptococci. J Clin Microbiol. 1980 Jul;12(1):27–31. doi: 10.1128/jcm.12.1.27-31.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]