Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1989 Sep;27(9):2105–2106. doi: 10.1128/jcm.27.9.2105-2106.1989

Comparison of media for screening of diarrheic stools for the recovery of Clostridium difficile.

P C Iwen 1, S J Booth 1, G L Woods 1
PMCID: PMC267748  PMID: 2778074

Abstract

Recoveries of Clostridium difficile from stool specimens by using three media, cycloserine-mannitol agar (M-CMA), cycloserine-mannitol-blood agar (M-CMBA), and cycloserine-cefoxitin agar (M-CCA), were compared. Of 321 clinical specimens, 37 yielded C. difficile. Thirty-four were positive on M-CCA, 21 were positive on M-CMA, and 20 were positive on M-CMBA. M-CCA recovered significantly more C. difficile than did M-CMBA or M-CMA.

Full text

PDF
2105

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aronsson B., Möllby R., Nord C. E. Antimicrobial agents and Clostridium difficile in acute enteric disease: epidemiological data from Sweden, 1980-1982. J Infect Dis. 1985 Mar;151(3):476–481. doi: 10.1093/infdis/151.3.476. [DOI] [PubMed] [Google Scholar]
  2. Bate G. Comparison of Minitek Anaerobe II, API An-Ident, and RapID ANA systems for identification of Clostridium difficile. Am J Clin Pathol. 1986 Jun;85(6):716–718. doi: 10.1093/ajcp/85.6.716. [DOI] [PubMed] [Google Scholar]
  3. Buchanan A. G. Selective enrichment broth culture for detection of Clostridium difficile and associated cytotoxin. J Clin Microbiol. 1984 Jul;20(1):74–76. doi: 10.1128/jcm.20.1.74-76.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Finegold S. M. Anaerobic infections and Clostridium difficile colitis emerging during antibacterial therapy. Scand J Infect Dis Suppl. 1986;49:160–164. [PubMed] [Google Scholar]
  5. George W. L., Sutter V. L., Citron D., Finegold S. M. Selective and differential medium for isolation of Clostridium difficile. J Clin Microbiol. 1979 Feb;9(2):214–219. doi: 10.1128/jcm.9.2.214-219.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gilligan P. H., McCarthy L. R., Genta V. M. Relative frequency of Clostridium difficile in patients with diarrheal disease. J Clin Microbiol. 1981 Jul;14(1):26–31. doi: 10.1128/jcm.14.1.26-31.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kelly M. T., Champagne S. G., Sherlock C. H., Noble M. A., Freeman H. J., Smith J. A. Commercial latex agglutination test for detection of Clostridium difficile-associated diarrhea. J Clin Microbiol. 1987 Jul;25(7):1244–1247. doi: 10.1128/jcm.25.7.1244-1247.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kuijper E. J., Oudbier J. H., Stuifbergen W. N., Jansz A., Zanen H. C. Application of whole-cell DNA restriction endonuclease profiles to the epidemiology of Clostridium difficile-induced diarrhea. J Clin Microbiol. 1987 Apr;25(4):751–753. doi: 10.1128/jcm.25.4.751-753.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lyerly D. M., Krivan H. C., Wilkins T. D. Clostridium difficile: its disease and toxins. Clin Microbiol Rev. 1988 Jan;1(1):1–18. doi: 10.1128/cmr.1.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mulligan M. E., Peterson L. R., Kwok R. Y., Clabots C. R., Gerding D. N. Immunoblots and plasmid fingerprints compared with serotyping and polyacrylamide gel electrophoresis for typing Clostridium difficile. J Clin Microbiol. 1988 Jan;26(1):41–46. doi: 10.1128/jcm.26.1.41-46.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. O'Farrell S., Wilks M., Nash J. Q., Tabaqchali S. A selective enrichment broth for the isolation of Clostridium difficile. J Clin Pathol. 1984 Jan;37(1):98–99. doi: 10.1136/jcp.37.1.98. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pantosti A., Cerquetti M., Gianfrilli P. M. Electrophoretic characterization of Clostridium difficile strains isolated from antibiotic-associated colitis and other conditions. J Clin Microbiol. 1988 Mar;26(3):540–543. doi: 10.1128/jcm.26.3.540-543.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Peach S. L., Borriello S. P., Gaya H., Barclay F. E., Welch A. R. Asymptomatic carriage of Clostridium difficile in patients with cystic fibrosis. J Clin Pathol. 1986 Sep;39(9):1013–1018. doi: 10.1136/jcp.39.9.1013. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Willey S. H., Bartlett J. G. Cultures for Clostridium difficile in stools containing a cytotoxin neutralized by Clostridium sordellii antitoxin. J Clin Microbiol. 1979 Dec;10(6):880–884. doi: 10.1128/jcm.10.6.880-884.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wilson K. H., Kennedy M. J., Fekety F. R. Use of sodium taurocholate to enhance spore recovery on a medium selective for Clostridium difficile. J Clin Microbiol. 1982 Mar;15(3):443–446. doi: 10.1128/jcm.15.3.443-446.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES