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. 1989 Sep;27(9):2123–2124. doi: 10.1128/jcm.27.9.2123-2124.1989

Mannose-resistant hemagglutination, enzyme-linked immunosorbent assay, and immune electron microscopy for detection of K99 fimbrial antigen in Escherichia coli from calves.

F Hernández 1, M Caballero 1, P Rivera 1, D Hird 1
PMCID: PMC267755  PMID: 2570792

Abstract

Mannose-resistant hemagglutination (MRHA) was evaluated for identification of Escherichia coli with K99 fimbriae. The sensitivity and specificity of MRHA, relative to the enzyme-linked immunosorbent assay, were 21 and 79%, respectively. Disagreement between the tests may have been due in part to separation of pili from the cells, with resultant enzyme-linked immunosorbent assay-positive, MRHA-negative tests. MRHA was not useful as the sole means for the identification of E. coli with K99 fimbriae.

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Selected References

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