Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1990 Apr;28(4):823–824. doi: 10.1128/jcm.28.4.823-824.1990

Biotype-specific probe for Vibrio cholerae serogroup O1.

R A Alm 1, P A Manning 1
PMCID: PMC267808  PMID: 2332478

Abstract

The O1 serogroup of Vibrio cholerae can be divided into two biotypes, El Tor and Classical. Current tests to distinguish between these biotypes are often difficult to interpret. On the basis of the difference in sequence of the hlyA gene in these biotypes, we have developed a simple probe that can easily and reliably differentiate between the two biotypes.

Full text

PDF
823

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alm R. A., Stroeher U. H., Manning P. A. Extracellular proteins of Vibrio cholerae: nucleotide sequence of the structural gene (hlyA) for the haemolysin of the haemolytic El Tor strain 017 and characterization of the hlyA mutation in the non-haemolytic classical strain 569B. Mol Microbiol. 1988 Jul;2(4):481–488. doi: 10.1111/j.1365-2958.1988.tb00054.x. [DOI] [PubMed] [Google Scholar]
  2. DE MOOR C. E. A NON-HAEMOLYTIC EL TOR VIBRIO AS THE CAUSE OF AN OUTBREAK OF PARACHOLERA IN WEST NEW GUINEA. THE EL TOR PROBLEM AND PANDEMIC PARACHOLERA IN THE WEST PACIFIC. Trop Geogr Med. 1963 Jun;15:97–107. [PubMed] [Google Scholar]
  3. Goldberg S. L., Murphy J. R. Cloning and characterization of the hemolysin determinants from Vibrio cholerae RV79(Hly+), RV79(Hly-), and 569B. J Bacteriol. 1985 Apr;162(1):35–41. doi: 10.1128/jb.162.1.35-41.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Rader A. E., Murphy J. R. Nucleotide sequences and comparison of the hemolysin determinants of Vibrio cholerae El Tor RV79(Hly+) and RV79(Hly-) and classical 569B(Hly-). Infect Immun. 1988 Jun;56(6):1414–1419. doi: 10.1128/iai.56.6.1414-1419.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Yamamoto K., Ichinose Y., Nakasone N., Tanabe M., Nagahama M., Sakurai J., Iwanaga M. Identity of hemolysins produced by Vibrio cholerae non-O1 and V. cholerae O1, biotype El Tor. Infect Immun. 1986 Mar;51(3):927–931. doi: 10.1128/iai.51.3.927-931.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES