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. 1990 May;28(5):1051–1053. doi: 10.1128/jcm.28.5.1051-1053.1990

Evaluation of a commercial microtiter system (MicroScan) using both frozen and freeze-dried panels for detection of high-level aminoglycoside resistance in Enterococcus spp.

S A Fuller 1, D E Low 1, A E Simor 1
PMCID: PMC267863  PMID: 2351721

Abstract

The MicroScan system was compared with agar dilution screen plates for the detection of high-level aminoglycoside resistance in 182 enterococcal isolates. Both the frozen Gram-Positive Combo Type 2 and the freeze-dried Type 5 panels were evaluated. The specificity of both panels for the detection of streptomycin and gentamicin resistance was 100%. However, the sensitivities for the detection of gentamicin and streptomycin resistance were 84 and 31%, respectively, for the Type 2 panels and 90 and 41%, respectively, for the Type 5 panels. The sensitivities of these panels for the detection of enterococcal high-level aminoglycoside resistance are inadequate for routine use.

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Selected References

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  1. Calderwood S. A., Wennersten C., Moellering R. C., Jr, Kunz L. J., Krogstad D. J. Resistance to six aminoglycosidic aminocyclitol antibiotics among enterococci: prevalence, evolution, and relationship to synergism with penicillin. Antimicrob Agents Chemother. 1977 Sep;12(3):401–405. doi: 10.1128/aac.12.3.401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Courvalin P., Carlier C., Collatz E. Plasmid-mediated resistance to aminocyclitol antibiotics in group D streptococci. J Bacteriol. 1980 Aug;143(2):541–551. doi: 10.1128/jb.143.2.541-551.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Eliopoulos G. M., Wennersten C., Zighelboim-Daum S., Reiszner E., Goldmann D., Moellering R. C., Jr High-level resistance to gentamicin in clinical isolates of Streptococcus (Enterococcus) faecium. Antimicrob Agents Chemother. 1988 Oct;32(10):1528–1532. doi: 10.1128/aac.32.10.1528. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gullberg R. M., Homann S. R., Phair J. P. Enterococcal bacteremia: analysis of 75 episodes. Rev Infect Dis. 1989 Jan-Feb;11(1):74–85. doi: 10.1093/clinids/11.1.74. [DOI] [PubMed] [Google Scholar]
  5. Hoffmann S. A., Moellering R. C., Jr The enterococcus: "putting the bug in our ears". Ann Intern Med. 1987 May;106(5):757–761. doi: 10.7326/0003-4819-106-5-757. [DOI] [PubMed] [Google Scholar]
  6. Ikeda D. P., Barry A. L., Andersen S. G. Emergence of Streptococcus faecalis isolates with high-level resistance to multiple aminocyclitol aminoglycosides. Diagn Microbiol Infect Dis. 1984 Jun;2(3):171–177. doi: 10.1016/0732-8893(84)90027-0. [DOI] [PubMed] [Google Scholar]
  7. Malone D. A., Wagner R. A., Myers J. P., Watanakunakorn C. Enterococcal bacteremia in two large community teaching hospitals. Am J Med. 1986 Oct;81(4):601–606. doi: 10.1016/0002-9343(86)90544-9. [DOI] [PubMed] [Google Scholar]
  8. Mederski-Samoraj B. D., Murray B. E. High-level resistance to gentamicin in clinical isolates of enterococci. J Infect Dis. 1983 Apr;147(4):751–757. doi: 10.1093/infdis/147.4.751. [DOI] [PubMed] [Google Scholar]
  9. Moellering R. C., Jr, Wennersten C., Weinberg A. N. Studies on antibiotic synergism against enterococci. I. Bacteriologic studies. J Lab Clin Med. 1971 May;77(5):821–828. [PubMed] [Google Scholar]
  10. Moellering R. C., Jr, Wennersten C., Weinstein A. J. Penicillin-tobramycin synergism against enterococci: a comparison with penicillin and gentamicin. Antimicrob Agents Chemother. 1973 Apr;3(4):526–529. doi: 10.1128/aac.3.4.526. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nachamkin I., Axelrod P., Talbot G. H., Fischer S. H., Wennersten C. B., Moellering R. C., Jr, MacGregor R. R. Multiply high-level-aminoglycoside-resistant enterococci isolated from patients in a university hospital. J Clin Microbiol. 1988 Jul;26(7):1287–1291. doi: 10.1128/jcm.26.7.1287-1291.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rosenthal S. L., Freundlich L. F. An aminoglycoside disk sensitivity test for use with enterococci. J Antimicrob Chemother. 1982 Nov;10(5):459–462. doi: 10.1093/jac/10.5.459. [DOI] [PubMed] [Google Scholar]
  13. Sahm D. F., Torres C. Effects of medium and inoculum variations on screening for high-level aminoglycoside resistance in Enterococcus faecalis. J Clin Microbiol. 1988 Feb;26(2):250–256. doi: 10.1128/jcm.26.2.250-256.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sahm D. F., Torres C. High-content aminoglycoside disks for determining aminoglycoside-penicillin synergy against Enterococcus faecalis. J Clin Microbiol. 1988 Feb;26(2):257–260. doi: 10.1128/jcm.26.2.257-260.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Spiegel C. A. Laboratory detection of high-level aminoglycoside-aminocyclitol resistance in Enterococcus spp. J Clin Microbiol. 1988 Nov;26(11):2270–2274. doi: 10.1128/jcm.26.11.2270-2274.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Watanakunakorn C. The prevalence of high-level aminoglycoside resistance among enterococci isolated from blood cultures during 1980-1988. J Antimicrob Chemother. 1989 Jul;24(1):63–68. doi: 10.1093/jac/24.1.63. [DOI] [PubMed] [Google Scholar]
  17. Zervos M. J., Kauffman C. A., Therasse P. M., Bergman A. G., Mikesell T. S., Schaberg D. R. Nosocomial infection by gentamicin-resistant Streptococcus faecalis. An epidemiologic study. Ann Intern Med. 1987 May;106(5):687–691. doi: 10.7326/0003-4819-106-5-687. [DOI] [PubMed] [Google Scholar]
  18. Zervos M. J., Patterson J. E., Edberg S., Pierson C., Kauffman C. A., Mikesell T. S., Schaberg D. R. Single-concentration broth microdilution test for detection of high-level aminoglycoside resistance in enterococci. J Clin Microbiol. 1987 Dec;25(12):2443–2444. doi: 10.1128/jcm.25.12.2443-2444.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

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