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. 1990 Jun;28(6):1098–1100. doi: 10.1128/jcm.28.6.1098-1100.1990

Amino acid requirements for growth of Chlamydia pneumoniae in cell cultures: growth enhancement by lysine or methionine depletion.

C C Kuo 1, J T Grayston 1
PMCID: PMC267883  PMID: 2116442

Abstract

Amino acid requirements for growth of two isolates of Chlamydia pneumoniae were studied and compared with those for one strain of Chlamydia trachomatis in a HeLa 229 cell culture. It was shown that among 13 amino acids in Eagle minimum essential medium, C. pneumoniae required all amino acids except lysine. A true requirement for arginine, isoleucine, leucine, threonine, and valine could not be determined because depletion of 100% of these amino acids caused cell detachment. Consequently, a requirement for these amino acids was based on 90% depletion. C. trachomatis biovar trachoma required all amino acids except threonine, which was indeterminable. Depletion of 100 and 90% of the lysine and 90 to 70% of the methionine was shown to enhance the growth of C. pneumoniae. This phenomenon was shown to be a property of C. pneumoniae because the effect of lysine and methionine reduction was also demonstrated in another human line, HL cells, and a mouse line, McCoy cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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