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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1990 Jun;28(6):1143–1146. doi: 10.1128/jcm.28.6.1143-1146.1990

Phenotypic conversion of Pseudomonas aeruginosa in cystic fibrosis.

M Fegan 1, P Francis 1, A C Hayward 1, G H Davis 1, J A Fuerst 1
PMCID: PMC267893  PMID: 2116444

Abstract

Pseudomonas aeruginosa strains isolated from cystic fibrosis patients were tested for production of exoenzymes, sensitivity to pooled normal human serum, and colony morphology. Strains isolated from patients exhibiting a severe form of the disease were seen to produce a decreased range of exoenzymes, to show an increase in their serum sensitivity, and to be predominantly mucoid in colonial character compared with strains isolated from patients with a milder form of the disease. These results suggest that P. aeruginosa undergoes phenotypic changes with respect to exoenzyme secretion, serum sensitivity, and colony form as the clinical condition of the cystic fibrosis patient changes.

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Selected References

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  1. Berry A., DeVault J. D., Chakrabarty A. M. High osmolarity is a signal for enhanced algD transcription in mucoid and nonmucoid Pseudomonas aeruginosa strains. J Bacteriol. 1989 May;171(5):2312–2317. doi: 10.1128/jb.171.5.2312-2317.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Costerton J. W. The etiology and persistence of cryptic bacterial infections: a hypothesis. Rev Infect Dis. 1984 Sep-Oct;6 (Suppl 3):S608–S616. doi: 10.1093/clinids/6.supplement_3.s608. [DOI] [PubMed] [Google Scholar]
  3. Davis P. B., di Sant'Agnese P. A. Diagnosis and treatment of cystic fibrosis. An update. Chest. 1984 Jun;85(6):802–809. doi: 10.1016/s0012-3692(16)62421-2. [DOI] [PubMed] [Google Scholar]
  4. Fick R. B., Jr, Naegel G. P., Squier S. U., Wood R. E., Gee J. B., Reynolds H. Y. Proteins of the cystic fibrosis respiratory tract. Fragmented immunoglobulin G opsonic antibody causing defective opsonophagocytosis. J Clin Invest. 1984 Jul;74(1):236–248. doi: 10.1172/JCI111407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Govan J. R., Harris G. S. Pseudomonas aeruginosa and cystic fibrosis: unusual bacterial adaptation and pathogenesis. Microbiol Sci. 1986 Oct;3(10):302–308. [PubMed] [Google Scholar]
  6. Hancock R. E., Mutharia L. M., Chan L., Darveau R. P., Speert D. P., Pier G. B. Pseudomonas aeruginosa isolates from patients with cystic fibrosis: a class of serum-sensitive, nontypable strains deficient in lipopolysaccharide O side chains. Infect Immun. 1983 Oct;42(1):170–177. doi: 10.1128/iai.42.1.170-177.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Heck L. W., Morihara K., Abrahamson D. R. Degradation of soluble laminin and depletion of tissue-associated basement membrane laminin by Pseudomonas aeruginosa elastase and alkaline protease. Infect Immun. 1986 Oct;54(1):149–153. doi: 10.1128/iai.54.1.149-153.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Heck L. W., Morihara K., McRae W. B., Miller E. J. Specific cleavage of human type III and IV collagens by Pseudomonas aeruginosa elastase. Infect Immun. 1986 Jan;51(1):115–118. doi: 10.1128/iai.51.1.115-118.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hingley S. T., Hastie A. T., Kueppers F., Higgins M. L., Weinbaum G., Shryock T. Effect of ciliostatic factors from Pseudomonas aeruginosa on rabbit respiratory cilia. Infect Immun. 1986 Jan;51(1):254–262. doi: 10.1128/iai.51.1.254-262.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hudson I., Phelan P. D. Are sex, age at diagnosis, or mode of presentation prognostic factors for cystic fibrosis? Pediatr Pulmonol. 1987 Sep-Oct;3(5):288–297. doi: 10.1002/ppul.1950030503. [DOI] [PubMed] [Google Scholar]
  11. Jagger K. S., Bahner D. R., Warren R. L. Protease phenotypes of Pseudomonas aeruginosa isolated from patients with cystic fibrosis. J Clin Microbiol. 1983 Jan;17(1):55–59. doi: 10.1128/jcm.17.1.55-59.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Janda J. M., Bottone E. J. Pseudomonas aeruginosa enzyme profiling: predictor of potential invasiveness and use as an epidemiological tool. J Clin Microbiol. 1981 Jul;14(1):55–60. doi: 10.1128/jcm.14.1.55-60.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Luzar M. A., Montie T. C. Avirulence and altered physiological properties of cystic fibrosis strains of Pseudomonas aeruginosa. Infect Immun. 1985 Nov;50(2):572–576. doi: 10.1128/iai.50.2.572-576.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ojeniyi B., Baek L., Høiby N. Polyagglutinability due to loss of O-antigenic determinants in Pseudomonas aeruginosa strains isolated from cystic fibrosis patients. Acta Pathol Microbiol Immunol Scand B. 1985 Feb;93(1):7–13. doi: 10.1111/j.1699-0463.1985.tb02844.x. [DOI] [PubMed] [Google Scholar]
  15. Pajdak E., Pituch-Noworolska A., Stankiewicz D. Interaction of elastase from Pseudomonas aeruginosa with polymorphonuclear leukocytes and serum. Arch Immunol Ther Exp (Warsz) 1985;33(4):499–505. [PubMed] [Google Scholar]
  16. Paul J. H., Deflaun M. F., Jeffrey W. H. Mechanisms of DNA utilization by estuarine microbial populations. Appl Environ Microbiol. 1988 Jul;54(7):1682–1688. doi: 10.1128/aem.54.7.1682-1688.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Penketh A., Pitt T., Roberts D., Hodson M. E., Batten J. C. The relationship of phenotype changes in Pseudomonas aeruginosa to the clinical condition of patients with cystic fibrosis. Am Rev Respir Dis. 1983 May;127(5):605–608. doi: 10.1164/arrd.1983.127.5.605. [DOI] [PubMed] [Google Scholar]
  18. Phillips I. Identification of Pseudomonas aeruginosa in the clinical laboratory. J Med Microbiol. 1969 Feb;2(1):9–16. doi: 10.1099/00222615-2-1-9. [DOI] [PubMed] [Google Scholar]
  19. Pier G. B., Desjardins D., Aguilar T., Barnard M., Speert D. P. Polysaccharide surface antigens expressed by nonmucoid isolates of Pseudomonas aeruginosa from cystic fibrosis patients. J Clin Microbiol. 1986 Aug;24(2):189–196. doi: 10.1128/jcm.24.2.189-196.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. SBARRA A. J., GILFILLAN R. F., BARDAWIL W. A. A plate assay for elastase. Nature. 1960 Oct 22;188:322–323. doi: 10.1038/188322b0. [DOI] [PubMed] [Google Scholar]
  21. SIERRA G. A simple method for the detection of lipolytic activity of micro-organisms and some observations on the influence of the contact between cells and fatty substrates. Antonie Van Leeuwenhoek. 1957;23(1):15–22. doi: 10.1007/BF02545855. [DOI] [PubMed] [Google Scholar]
  22. Schiller N. L., Hatch R. A., Joiner K. A. Complement activation and C3 binding by serum-sensitive and serum-resistant strains of Pseudomonas aeruginosa. Infect Immun. 1989 Jun;57(6):1707–1713. doi: 10.1128/iai.57.6.1707-1713.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Schultz D. R., Miller K. D. Elastase of Pseudomonas aeruginosa: inactivation of complement components and complement-derived chemotactic and phagocytic factors. Infect Immun. 1974 Jul;10(1):128–135. doi: 10.1128/iai.10.1.128-135.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sherbrock-Cox V., Russell N. J., Gacesa P. The purification and chemical characterisation of the alginate present in extracellular material produced by mucoid strains of Pseudomonas aeruginosa. Carbohydr Res. 1984 Dec 15;135(1):147–154. doi: 10.1016/0008-6215(84)85012-0. [DOI] [PubMed] [Google Scholar]
  25. Simpson J. A., Smith S. E., Dean R. T. Alginate inhibition of the uptake of Pseudomonas aeruginosa by macrophages. J Gen Microbiol. 1988 Jan;134(1):29–36. doi: 10.1099/00221287-134-1-29. [DOI] [PubMed] [Google Scholar]
  26. Sokol P. A., Ohman D. E., Iglewski B. H. A more sensitive plate assay for detection of protease production by Pseudomanas aeruginosa. J Clin Microbiol. 1979 Apr;9(4):538–540. doi: 10.1128/jcm.9.4.538-540.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wood R. E., Boat T. F., Doershuk C. F. Cystic fibrosis. Am Rev Respir Dis. 1976 Jun;113(6):833–878. doi: 10.1164/arrd.1976.113.6.833. [DOI] [PubMed] [Google Scholar]
  28. Woods D. E., Bass J. A., Johanson W. G., Jr, Straus D. C. Role of adherence in the pathogenesis of Pseudomonas aeruginosa lung infection in cystic fibrosis patients. Infect Immun. 1980 Dec;30(3):694–699. doi: 10.1128/iai.30.3.694-699.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Woods D. E., Cryz S. J., Friedman R. L., Iglewski B. H. Contribution of toxin A and elastase to virulence of Pseudomonas aeruginosa in chronic lung infections of rats. Infect Immun. 1982 Jun;36(3):1223–1228. doi: 10.1128/iai.36.3.1223-1228.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

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