Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1990 Jun;28(6):1147–1150. doi: 10.1128/jcm.28.6.1147-1150.1990

Human malignant melanoma cell line (HMV-II) for isolation of influenza C and parainfluenza viruses.

H Moriuchi 1, T Oshima 1, H Nishimura 1, K Nakamura 1, N Katsushima 1, Y Numazaki 1
PMCID: PMC267894  PMID: 2166075

Abstract

HMV-II, a human malignant melanoma cell line, was compared with other cell lines (MDCK, Vero, and LLC-MK2) and primary cultures of monkey kidney (PMK) cells for the isolation and quantification of influenza and parainfluenza viruses. HMV-II cells were superior to MDCK and LLC-MK2 cells in quantification of the influenza C virus and were used successfully in the isolation of the virus from clinical specimens. The HMV-II cell line was also more sensitive for isolating parainfluenza viruses from clinical specimens than were Vero and PMK cells; there was, however, no significant difference in the quantification of the viruses among these cultures. As far as influenza A and B viruses were concerned, the HMV-II cell line was significantly less sensitive than MDCK cells, and no virus was isolated from clinical specimens with HMV-II cells. Thus, HMV-II cells are useful for the isolation of influenza C and parainfluenza viruses as an alternative to embryonated hen's eggs and PMK cells.

Full text

PDF
1147

Images in this article

1148Image
on p.1148

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chakraverty P. The detection and multiplication of influenza C virus in tissue culture. J Gen Virol. 1974 Dec;25(3):421–425. doi: 10.1099/0022-1317-25-3-421. [DOI] [PubMed] [Google Scholar]
  2. Frank A. L., Couch R. B., Griffis C. A., Baxter B. D. Comparison of different tissue cultures for isolation and quantitation of influenza and parainfluenza viruses. J Clin Microbiol. 1979 Jul;10(1):32–36. doi: 10.1128/jcm.10.1.32-36.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Homma M., Ohyama S., Katagiri S. Age distribution of the antibody to type C influenza virus. Microbiol Immunol. 1982;26(7):639–642. doi: 10.1111/mim.1982.26.7.639. [DOI] [PubMed] [Google Scholar]
  4. Ito H., Morimoto Y., Iwase I., Doi Y., Sanpe T. Effect of trypsin on viral susceptibility of Vero cell cultures--cercopithecus kidney line. Jpn J Med Sci Biol. 1970 Aug;23(4):227–235. doi: 10.7883/yoken1952.23.227. [DOI] [PubMed] [Google Scholar]
  5. Katagiri S., Ohizumi A., Homma M. An outbreak of type C influenza in a children's home. J Infect Dis. 1983 Jul;148(1):51–56. doi: 10.1093/infdis/148.1.51. [DOI] [PubMed] [Google Scholar]
  6. MOGABGAB W. J. Influenza C virus in monkey kidney tissue cultures. J Bacteriol. 1962 Jan;83:209–210. doi: 10.1128/jb.83.1.209-210.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Meguro H., Bryant J. D., Torrence A. E., Wright P. F. Canine kidney cell line for isolation of respiratory viruses. J Clin Microbiol. 1979 Feb;9(2):175–179. doi: 10.1128/jcm.9.2.175-179.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Morimoto Y., Doi Y., Ito H. Effect of trypsin on reproduction of type 4 parainfluenza virus in Vero cell cultures under fluid overlay. Jpn J Med Sci Biol. 1970 Feb;23(1):1–11. doi: 10.7883/yoken1952.23.1. [DOI] [PubMed] [Google Scholar]
  9. Nakamura T., Seki S., Matsubara O., Ito S., Kasuga T. Specific incorporation of 4-S-cysteinylphenol into human melanoma cells. J Invest Dermatol. 1988 May;90(5):725–728. doi: 10.1111/1523-1747.ep12560926. [DOI] [PubMed] [Google Scholar]
  10. Nerome K., Ishida M. The multiplication of an influenza C virus in an established line of canine kidney (MDCK) cells. J Gen Virol. 1978 Apr;39(1):179–181. doi: 10.1099/0022-1317-39-1-179. [DOI] [PubMed] [Google Scholar]
  11. Nerome K., Nakayama M., Ishida M. Established cell line sensitive to influenza C virus. J Gen Virol. 1979 Apr;43(1):257–259. doi: 10.1099/0022-1317-43-1-257. [DOI] [PubMed] [Google Scholar]
  12. Nishimura H., Sugawara K., Kitame F., Nakamura K., Katsushima N., Moriuchi H., Numazaki Y. A human melanoma cell line highly susceptible to influenza C virus. J Gen Virol. 1989 Jul;70(Pt 7):1653–1661. doi: 10.1099/0022-1317-70-7-1653. [DOI] [PubMed] [Google Scholar]
  13. Numazaki Y., Oshima T., Ohmi A., Tanaka A., Oizumi Y., Komatsu S., Takagi T., Karahashi M., Ishida N. A microplate method for isolation of viruses from infants and children with acute respiratory infections. Microbiol Immunol. 1987;31(11):1085–1095. doi: 10.1111/j.1348-0421.1987.tb01340.x. [DOI] [PubMed] [Google Scholar]
  14. Numazaki Y., Shigeta S., Ishida N. Studies on parainfluenza virus infections among infants and children in Sendai. I. Isolation and identification methods of parainfluenza viruses. Jpn J Microbiol. 1968 Sep;12(3):275–281. doi: 10.1111/j.1348-0421.1968.tb00396.x. [DOI] [PubMed] [Google Scholar]
  15. Sugita K., Maru M., Sato K. A sensitive plaque assay for Sendai virus in an established line of monkey kidney cells. Jpn J Microbiol. 1974 May;18(3):262–264. doi: 10.1111/j.1348-0421.1974.tb00955.x. [DOI] [PubMed] [Google Scholar]
  16. TAKEMOTO K. K., LYNT R. K., ROWE W. P., HUEBNER R. J., BELL J. A., MELLIN G. W., DAVIS D. J. Primary isolation of influenza A, B, and C viruses in monkey kidney tissue cultures. Proc Soc Exp Biol Med. 1955 Jun;89(2):308–311. doi: 10.3181/00379727-89-21794. [DOI] [PubMed] [Google Scholar]
  17. Tobita K., Sugiura A., Enomote C., Furuyama M. Plaque assay and primary isolation of influenza A viruses in an established line of canine kidney cells (MDCK) in the presence of trypsin. Med Microbiol Immunol. 1975 Dec 30;162(1):9–14. doi: 10.1007/BF02123572. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES