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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1990 Jun;28(6):1147–1150. doi: 10.1128/jcm.28.6.1147-1150.1990

Human malignant melanoma cell line (HMV-II) for isolation of influenza C and parainfluenza viruses.

H Moriuchi 1, T Oshima 1, H Nishimura 1, K Nakamura 1, N Katsushima 1, Y Numazaki 1
PMCID: PMC267894  PMID: 2166075

Abstract

HMV-II, a human malignant melanoma cell line, was compared with other cell lines (MDCK, Vero, and LLC-MK2) and primary cultures of monkey kidney (PMK) cells for the isolation and quantification of influenza and parainfluenza viruses. HMV-II cells were superior to MDCK and LLC-MK2 cells in quantification of the influenza C virus and were used successfully in the isolation of the virus from clinical specimens. The HMV-II cell line was also more sensitive for isolating parainfluenza viruses from clinical specimens than were Vero and PMK cells; there was, however, no significant difference in the quantification of the viruses among these cultures. As far as influenza A and B viruses were concerned, the HMV-II cell line was significantly less sensitive than MDCK cells, and no virus was isolated from clinical specimens with HMV-II cells. Thus, HMV-II cells are useful for the isolation of influenza C and parainfluenza viruses as an alternative to embryonated hen's eggs and PMK cells.

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Selected References

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  1. Chakraverty P. The detection and multiplication of influenza C virus in tissue culture. J Gen Virol. 1974 Dec;25(3):421–425. doi: 10.1099/0022-1317-25-3-421. [DOI] [PubMed] [Google Scholar]
  2. Frank A. L., Couch R. B., Griffis C. A., Baxter B. D. Comparison of different tissue cultures for isolation and quantitation of influenza and parainfluenza viruses. J Clin Microbiol. 1979 Jul;10(1):32–36. doi: 10.1128/jcm.10.1.32-36.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Homma M., Ohyama S., Katagiri S. Age distribution of the antibody to type C influenza virus. Microbiol Immunol. 1982;26(7):639–642. doi: 10.1111/mim.1982.26.7.639. [DOI] [PubMed] [Google Scholar]
  4. Ito H., Morimoto Y., Iwase I., Doi Y., Sanpe T. Effect of trypsin on viral susceptibility of Vero cell cultures--cercopithecus kidney line. Jpn J Med Sci Biol. 1970 Aug;23(4):227–235. doi: 10.7883/yoken1952.23.227. [DOI] [PubMed] [Google Scholar]
  5. Katagiri S., Ohizumi A., Homma M. An outbreak of type C influenza in a children's home. J Infect Dis. 1983 Jul;148(1):51–56. doi: 10.1093/infdis/148.1.51. [DOI] [PubMed] [Google Scholar]
  6. MOGABGAB W. J. Influenza C virus in monkey kidney tissue cultures. J Bacteriol. 1962 Jan;83:209–210. doi: 10.1128/jb.83.1.209-210.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Meguro H., Bryant J. D., Torrence A. E., Wright P. F. Canine kidney cell line for isolation of respiratory viruses. J Clin Microbiol. 1979 Feb;9(2):175–179. doi: 10.1128/jcm.9.2.175-179.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Morimoto Y., Doi Y., Ito H. Effect of trypsin on reproduction of type 4 parainfluenza virus in Vero cell cultures under fluid overlay. Jpn J Med Sci Biol. 1970 Feb;23(1):1–11. doi: 10.7883/yoken1952.23.1. [DOI] [PubMed] [Google Scholar]
  9. Nakamura T., Seki S., Matsubara O., Ito S., Kasuga T. Specific incorporation of 4-S-cysteinylphenol into human melanoma cells. J Invest Dermatol. 1988 May;90(5):725–728. doi: 10.1111/1523-1747.ep12560926. [DOI] [PubMed] [Google Scholar]
  10. Nerome K., Ishida M. The multiplication of an influenza C virus in an established line of canine kidney (MDCK) cells. J Gen Virol. 1978 Apr;39(1):179–181. doi: 10.1099/0022-1317-39-1-179. [DOI] [PubMed] [Google Scholar]
  11. Nerome K., Nakayama M., Ishida M. Established cell line sensitive to influenza C virus. J Gen Virol. 1979 Apr;43(1):257–259. doi: 10.1099/0022-1317-43-1-257. [DOI] [PubMed] [Google Scholar]
  12. Nishimura H., Sugawara K., Kitame F., Nakamura K., Katsushima N., Moriuchi H., Numazaki Y. A human melanoma cell line highly susceptible to influenza C virus. J Gen Virol. 1989 Jul;70(Pt 7):1653–1661. doi: 10.1099/0022-1317-70-7-1653. [DOI] [PubMed] [Google Scholar]
  13. Numazaki Y., Oshima T., Ohmi A., Tanaka A., Oizumi Y., Komatsu S., Takagi T., Karahashi M., Ishida N. A microplate method for isolation of viruses from infants and children with acute respiratory infections. Microbiol Immunol. 1987;31(11):1085–1095. doi: 10.1111/j.1348-0421.1987.tb01340.x. [DOI] [PubMed] [Google Scholar]
  14. Numazaki Y., Shigeta S., Ishida N. Studies on parainfluenza virus infections among infants and children in Sendai. I. Isolation and identification methods of parainfluenza viruses. Jpn J Microbiol. 1968 Sep;12(3):275–281. doi: 10.1111/j.1348-0421.1968.tb00396.x. [DOI] [PubMed] [Google Scholar]
  15. Sugita K., Maru M., Sato K. A sensitive plaque assay for Sendai virus in an established line of monkey kidney cells. Jpn J Microbiol. 1974 May;18(3):262–264. doi: 10.1111/j.1348-0421.1974.tb00955.x. [DOI] [PubMed] [Google Scholar]
  16. TAKEMOTO K. K., LYNT R. K., ROWE W. P., HUEBNER R. J., BELL J. A., MELLIN G. W., DAVIS D. J. Primary isolation of influenza A, B, and C viruses in monkey kidney tissue cultures. Proc Soc Exp Biol Med. 1955 Jun;89(2):308–311. doi: 10.3181/00379727-89-21794. [DOI] [PubMed] [Google Scholar]
  17. Tobita K., Sugiura A., Enomote C., Furuyama M. Plaque assay and primary isolation of influenza A viruses in an established line of canine kidney cells (MDCK) in the presence of trypsin. Med Microbiol Immunol. 1975 Dec 30;162(1):9–14. doi: 10.1007/BF02123572. [DOI] [PubMed] [Google Scholar]

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