Abstract
Overlapping octapeptides from the amino acid sequence of the adherence protein of Mycoplasma pneumoniae were synthesized and used as the antigen in an enzyme-linked immunosorbent assay with serum samples from M. pneumoniae-infected patients. Of a sequence of 338 amino acids positioned between leucine 801 and leucine 1139, only two regions with immunodominant continuous epitopes were detected. The immunoglobulin G and immunoglobulin M antibodies of child and adult patients reacted especially with the NH2-(810)-W-I-G-N-G-Y-R-Y peptide but also reacted with the NH2-(1124)-F-T-D-F-V-K-P-R peptide.
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Selected References
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- Baseman J. B., Cole R. M., Krause D. C., Leith D. K. Molecular basis for cytadsorption of Mycoplasma pneumoniae. J Bacteriol. 1982 Sep;151(3):1514–1522. doi: 10.1128/jb.151.3.1514-1522.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baseman J. B., Dallo S. F., Tully J. G., Rose D. L. Isolation and characterization of Mycoplasma genitalium strains from the human respiratory tract. J Clin Microbiol. 1988 Nov;26(11):2266–2269. doi: 10.1128/jcm.26.11.2266-2269.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bredt W., Kleinmann B., Jacobs E. Antibodies in the sera of Mycoplasma pneumoniae-infected patients against proteins of Mycoplasma genitalium and other mycoplasmas of man. Zentralbl Bakteriol Mikrobiol Hyg A. 1987 Aug;266(1-2):32–42. doi: 10.1016/s0176-6724(87)80018-4. [DOI] [PubMed] [Google Scholar]
- Chou P. Y., Fasman G. D. Prediction of beta-turns. Biophys J. 1979 Jun;26(3):367–383. doi: 10.1016/S0006-3495(79)85259-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dallo S. F., Chavoya A., Su C. J., Baseman J. B. DNA and protein sequence homologies between the adhesins of Mycoplasma genitalium and Mycoplasma pneumoniae. Infect Immun. 1989 Apr;57(4):1059–1065. doi: 10.1128/iai.57.4.1059-1065.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Feldner J., Göbel U., Bredt W. Mycoplasma pneumoniae adhesin localized to tip structure by monoclonal antibody. Nature. 1982 Aug 19;298(5876):765–767. doi: 10.1038/298765a0. [DOI] [PubMed] [Google Scholar]
- Foy H. M., Kenny G. E., Cooney M. K., Allan I. D. Long-term epidemiology of infections with Mycoplasma pneumoniae. J Infect Dis. 1979 Jun;139(6):681–687. doi: 10.1093/infdis/139.6.681. [DOI] [PubMed] [Google Scholar]
- Geysen H. M., Meloen R. H., Barteling S. J. Use of peptide synthesis to probe viral antigens for epitopes to a resolution of a single amino acid. Proc Natl Acad Sci U S A. 1984 Jul;81(13):3998–4002. doi: 10.1073/pnas.81.13.3998. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hayflick L. Tissue cultures and mycoplasmas. Tex Rep Biol Med. 1965 Jun;23(Suppl):285+–285+. [PubMed] [Google Scholar]
- Hopp T. P., Woods K. R. Prediction of protein antigenic determinants from amino acid sequences. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3824–3828. doi: 10.1073/pnas.78.6.3824. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hu P. C., Cole R. M., Huang Y. S., Graham J. A., Gardner D. E., Collier A. M., Clyde W. A., Jr Mycoplasma pneumoniae infection: role of a surface protein in the attachment organelle. Science. 1982 Apr 16;216(4543):313–315. doi: 10.1126/science.6801766. [DOI] [PubMed] [Google Scholar]
- Inamine J. M., Denny T. P., Loechel S., Schaper U., Huang C. H., Bott K. F., Hu P. C. Nucleotide sequence of the P1 attachment-protein gene of Mycoplasma pneumoniae. Gene. 1988 Apr 29;64(2):217–229. doi: 10.1016/0378-1119(88)90337-x. [DOI] [PubMed] [Google Scholar]
- Jacobs E., Bennewitz A., Bredt W. Reaction pattern of human anti-Mycoplasma pneumoniae antibodies in enzyme-linked immunosorbent assays and immunoblotting. J Clin Microbiol. 1986 Mar;23(3):517–522. doi: 10.1128/jcm.23.3.517-522.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jacobs E., Buchholz A., Kleinmann B., Bredt W. Use of adherence protein of Mycoplasma pneumoniae as antigen for enzyme-linked immunosorbent assay (ELISA). Isr J Med Sci. 1987 Jun;23(6):709–712. [PubMed] [Google Scholar]
- Jacobs E., Clad A. Electroelution of fixed and stained membrane proteins from preparative sodium dodecyl sulfate-polyacrylamide gels into a membrane trap. Anal Biochem. 1986 May 1;154(2):583–589. doi: 10.1016/0003-2697(86)90033-3. [DOI] [PubMed] [Google Scholar]
- Jacobs E., Fuchte K., Bredt W. A 168-kilodalton protein of Mycoplasma pneumoniae used as antigen in a dot enzyme-linked immunosorbent assay. Eur J Clin Microbiol. 1986 Aug;5(4):435–440. doi: 10.1007/BF02075700. [DOI] [PubMed] [Google Scholar]
- Jacobs E., Fuchte K., Bredt W. Isolation of the adherence protein of Mycoplasma pneumoniae by fractionated solubilization and size exclusion chromatography. Biol Chem Hoppe Seyler. 1988 Dec;369(12):1295–1299. doi: 10.1515/bchm3.1988.369.2.1295. [DOI] [PubMed] [Google Scholar]
- Jacobs E., Gerstenecker B., Mader B., Huang C. H., Hu P. C., Halter R., Bredt W. Binding sites of attachment-inhibiting monoclonal antibodies and antibodies from patients on peptide fragments of the Mycoplasma pneumoniae adhesin. Infect Immun. 1989 Mar;57(3):685–688. doi: 10.1128/iai.57.3.685-688.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Murphy T. F., Henderson F. W., Clyde W. A., Jr, Collier A. M., Denny F. W. Pneumonia: an eleven-year study in a pediatric practice. Am J Epidemiol. 1981 Jan;113(1):12–21. doi: 10.1093/oxfordjournals.aje.a113061. [DOI] [PubMed] [Google Scholar]
- Rose G. D., Gierasch L. M., Smith J. A. Turns in peptides and proteins. Adv Protein Chem. 1985;37:1–109. doi: 10.1016/s0065-3233(08)60063-7. [DOI] [PubMed] [Google Scholar]
- SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]
- Su C. J., Tryon V. V., Baseman J. B. Cloning and sequence analysis of cytadhesin P1 gene from Mycoplasma pneumoniae. Infect Immun. 1987 Dec;55(12):3023–3029. doi: 10.1128/iai.55.12.3023-3029.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]